Botanical Information Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (under development)
| Synonyms | Acanthopanax asperatus Franch. & Sav. |
| Acanthopanax senticosus (Rupr. & Maxim.) Harms | |
| Acanthopanax senticosus f. inermis (Kom.) Harms | |
| Acanthopanax senticosus f. subinermis (Regel) Harms | |
| Acanthopanax senticosus var. brevistamineus S.F.Gu | |
| Acanthopanax senticosus var. subinermis (Regel) Kitag. | |
| Eleutherococcus asperatus (Franch. & Sav.) Koidz. | |
| Eleutherococcus koreanus Nakai | |
| Eleutherococcus senticosus f. inermis Kom. | |
| Eleutherococcus senticosus f. subinermis Regel | |
| Hedera senticosa Rupr. & Maxim. | |
| Common name (en) | Siberian ginseng |
| Common name (ar) | بناقنثة لاسعة |
| Common name (de) | Borstige Taigawurzel |
| Common name (es) | Ginseng siberiano |
| Common name (fr) | Ginseng de Sibérie |
| Common name (it) | Ginseng siberiano |
| Common name (ja) | エゾウコギ |
| Common name (ko) | 가시오갈피 |
| Common name (ru) | Элеутерококк колючий |
| Common name (vi) | Sâm Tây Bá Lợi Á |
| Common name (zh) | 刺五加 |
| Sample preparation | Mix 1 g of powdered sample with 5 mL of ethanol, water 1: 1 (v/v) and sonicate for 10 min, then centrifuge or filter the solutions and use the supernatant/filtrate as test solution |
| Mobile phase | Chloroform, methanol, water 70:30:4 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| System Suitability Test (SST) |
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| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of alcohol (50% v/v) and boil under reflux for 1 h. Cool and filter. Evaporate the filtrate to dryness on a water-bath. Dissolve the residue in 2.5 mL of a mixture of 5 volumes of water and 20 volumes of alcohol (50% v/v) and filter |
| Mobile phase | Water, methanol, methylene chloride 4:30:70 (v/v/v) |
| Derivatization | Spray with anisaldehyde solution and examine in daylight while heating at 100-105 °C for 5-10 min |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 15-mL centrifuge tube, then add 5 mL of ethanol (50%). Sonicate (140 W) the mixture for 10 min. Centrifuge at about 2800 × g for 10 min. Filter through a 0.45-µm nylon filter |
| Mobile phase | Dichloromethane, methanol, water 10:2:0.1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | Weigh 1 g of pulverized drug, add 10 mL of methanol, shake thoroughly, filter and evaporate the filtrate to dryness. Dissolve the residue in 1 mL of methanol and use this solution as the test solution |
| Mobile phase | Chloroform, methanol, water 70:30:4 (v/v/v) |
| Derivatization | Spray with sulfuric acid and heat at 105°C |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | To 5 g of the powder, add 50 mL of 75% ethanol, heat under reflux for 1h, filter and evaporate the filtrate to dryness, dissolve the residue with 10 mL water, extract by shaking with two 5-mL quantities of chloroform, combine the chloroform solutions, evaporate to dryness, dissolve the residue in 1 mL of methanol as the test solution |
| Mobile phase | Chloroform, methanol 19:1 (v/v) |
| Derivatization | No derivatization |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | About 2.5 g of raw material, crushed to the size of the particles passing through a sieve with holes of 0.5 mm in size, placed in a conical flask with a thin section with a capacity of 100 ml, add 15 ml of a mixture of alcohol 96% - water (1: 1) and refluxed in a water bath for 30 min. After cooling to room temperature, the resulting extract filtered through a paper filter |
| Mobile phase | Chloroform, methanol, and water 70:30:4 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 100°C for 3 min |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | Transfer about 1 g of finely powdered Eleuthero Root and Rhizome to a centrifuge tube, add 5 mL of alcohol (50% v/v), and mix well. Sonicate for 10 min. Centrifuge or filter the solution, and use the supernatant or the filtrate |
| Mobile phase | Chloroform, methanol, and water 35:15:2 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid solution in methanol and examine in daylight while heating at 100°C for 5 min |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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