Botanical Information Eucommia ulmoides Oliv. (procuring samples)

Synonyms Eucommia ulmoides var. yunnanensis H.Lév.
Common name (de) Chinesischer Guttaperchabaum
Common name (fr) Arbre à gomme
Common name (ja) トチュウ
Common name (ko) 두충
Common name (ru) Эвкоммия
Common name (th) โต๋วต๋ง
Common name (vi) Đỗ trọng
Common name (zh) 杜仲

Sample preparation To 1 g of the powdered herbal drug add 10 mL of methylene chloride. Sonicate for 10 min. Discard the liquid phase and repeat the extraction with another 10 mL of methylene chloride. Discard the liquid phase again. Dry the residue in air. Add 7 mL of methanol. Sonicate in a centrifuge tube at 60 °C for 20 min. Centrifuge; use the supernatant
Mobile phase Formic acid, ethyl acetate, toluene 1:35:65 (v/v/v)
Derivatization Treat with anisaldehyde solution and heat at 100-105°C for 5 min
Sample preparation Weigh accurately1 g of the powder in a stoppered conical flask, add accurately 25 mL of 50% methanol, weigh, heat on a water bath for 30 min, cool, weigh again, replenish the loss of the weigh with 50% methanol, mix well, filter and use the successive filtrate
Mobile phase Ethyl acetate, formic acid, water 7:2.5:2.5 (v/v/v)
Derivatization No derivatization