Botanical Information Eucommia ulmoides Oliv. (procuring samples)
| Synonyms | Eucommia ulmoides var. yunnanensis H.Lév. |
| Common name (de) | Chinesischer Guttaperchabaum |
| Common name (fr) | Arbre à gomme |
| Common name (ja) | トチュウ |
| Common name (ko) | 두충 |
| Common name (ru) | Эвкоммия |
| Common name (th) | โต๋วต๋ง |
| Common name (vi) | Đỗ trọng |
| Common name (zh) | 杜仲 |
| Sample preparation | To 1 g of the powdered herbal drug add 10 mL of methylene chloride. Sonicate for 10 min. Discard the liquid phase and repeat the extraction with another 10 mL of methylene chloride. Discard the liquid phase again. Dry the residue in air. Add 7 mL of methanol. Sonicate in a centrifuge tube at 60 °C for 20 min. Centrifuge; use the supernatant |
| Mobile phase | Formic acid, ethyl acetate, toluene 1:35:65 (v/v/v) |
| Derivatization | Treat with anisaldehyde solution and heat at 100-105°C for 5 min |
| Sample preparation | Weigh 2.5 g of the powdered sample and place it in a 100-mL conical flask, then add 30 mL of methanol. Sonicate (560 W) the mixture for 30 min. Filter and evaporate the filtrate to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 20 mL of water. Transfer the aqueous solution to a separator funnel. Extract the solution with 50 mL of dichloromethane and discard the dichloromethane layer. Extract the aqueous layer with 50 mL of 1-butanol. Evaporate the 1-butanol extract to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 1 mL of methanol |
| Mobile phase | Dichloromethane, methanol, formic acid 3:1:0.1 (v/v/v) |
| Derivatization | Spray with 20% sulfuric acid in water and heat the plate at 120°C until the spot appear |
| Sample preparation | Weigh accurately1 g of the powder in a stoppered conical flask, add accurately 25 mL of 50% methanol, weigh, heat on a water bath for 30 min, cool, weigh again, replenish the loss of the weigh with 50% methanol, mix well, filter and use the successive filtrate |
| Mobile phase | Ethyl acetate, formic acid, water 7:2.5:2.5 (v/v/v) |
| Derivatization | No derivatization |