Botanical Information Dioscorea nipponica Makino (procuring samples)
Synonyms | Dioscorea acerifolia var. rosthornii Diels |
Dioscorea giraldii R.Knuth | |
Dioscorea nipponica f. jamesii (Prain & Burkill) Kitag. | |
Dioscorea nipponica subsp. rosthornii (Diels) C.T.Ting | |
Dioscorea nipponica var. jamesii Prain & Burkill | |
Dioscorea nipponica var. pubescens Nakai | |
Dioscorea nipponica var. rosthornii (Diels) Prain & Burkill | |
Common name (en) | Uchiwa Yam |
Common name (zh) | 穿山龙 |
Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of methanol. Sonicate for 10 min. Centrifuge and use the supernatant |
Mobile phase | Water, methanol, methylene chloride 10:50:64 (v/v/v) |
Derivatization | Treat with anisaldehyde solution, heat at 100 °C for 3 min, allow to cool for 10 min and examine |
Sample preparation | Weigh 0.5 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 20 mL of ethanol (65%). Sonicate (350 W) the mixture for 30 min. Centrifuge at about 4500 × g for 10 min. Filter through a 0.45-µm nylon filter |
Mobile phase | Use the upper layer of the mixture water, n-butanol, ethyl acetate 5:4:1 (v/v/v) |
Derivatization | Spray with 10% sulfuric acid in ethanol, then heat the plate at 105°C for 3 min |
Sample preparation | To 0.5 g of the powder, add 25 mL of methanol, ultrasonicate for 30 min, filter and evaporate the filtrate to dryness. Dissolve the residue in 20 mL of a 3 mol/L solution of hydrochloric acid, heat to hydrolyze on a water bath for 30 min, cool, add 30 mL of chloroform, heat under reflux for 15 min, filter and evaporate the filtrate to dryness. Dissolve the residue in 2 mL of a mixture of chloroform, methanol 1:1 as the test solution |
Mobile phase | Chloroform, methanol 20:0.2 (v/v) |
Derivatization | Spray with 10% solution of phosphomolybdic acid in ethanol, heat at 105°C for 10 min |