Botanical Information Tinospora crispa (L.) Hook. f. & Thomson (under development)
Synonyms | Chasmanthera crispa (L.) Baill. |
Cocculus bantamensis Blume | |
Cocculus crispus (L.) DC. | |
Cocculus rimosus Blume | |
Cocculus verrucosus (Roxb.) Wall. | |
Menispermum bantamense (Blume) Spreng. | |
Menispermum crispum L. | |
Menispermum rimosum (Blume) Spreng. | |
Menispermum tuberculatum Lam. | |
Menispermum verrucosum Roxb. | |
Nephroia bantamensis (Blume) Miers | |
Tinospora gibbericaulis Hand.-Mazz. | |
Tinospora mastersii Diels | |
Tinospora rumphii Boerl. | |
Tinospora thorelii Gagnep. | |
Tinospora tuberculata (Lam.) Beumée ex K.Heyne Nutt. | |
Tinospora verrucosa (Roxb.) W.Theob. | |
Common name (en) | Heartleaf moonseed |
Sample preparation | Extract about 0.5 g of the T. crispa dried stem with 5 mL of methanol. Sonicate the solution for 15 min and filter |
Mobile phase | Chloroform, methanol, water 8:2:0.2 (v/v/v) |
Derivatization | Spray with 10% solution of sulfuric acid in ethanol, and heat at 105°C until the colors of the zones become visible |
Sample preparation | Extract about 0.5 g of the T. crispa dried stem with 5 mL of methanol. Sonicate the solution for 15 min and filter |
Mobile phase | Chloroform, methanol, water 8:2:0.2 (v/v/v) |
Derivatization | Spray with 10% sulfuric acid in ethanol, and heat at 105°C until the colors of the zones become visible |
Specific marker(s) | syringin |
Sample preparation | Warm, while shaking, 1 g of the powdered sample in 10 mL of methanol on a water bath at 60°C for 10 min. Filter and evaporate to 2 mL |
Mobile phase | Hexane, ethyl acetate, glacial acetic acid 75:25:1 (v/v/v) |
Derivatization | Spray with a 10% w/v solution of phosphomolybdic acid in ethanol, and heat to 100°C for 5 to 10 min |
Sample preparation | Warm, while shaking, 1 g of the powdered sample in 10 mL of methanol on a water bath at 60° for 10 min. Filter and evaporate to 2 mL |
Mobile phase | Chloroform, methanol, strong ammonia solution 75:20:5 (v/v/v) |
Derivatization | spray with acidic potassium iodobismuthate |
Sample preparation | Extract 1 g of powdered sample with 10 mL methanol and 1 mL 18M ammonia by refluxing in a water bath for 10-15 min, cool and filter |
Mobile phase | Chloroform, methanol, strong ammonia solution 75:20:5 (v/v/v) |
Derivatization | spray with iodoplatinate |