Botanical Information Schisandra chinensis (Turcz.) Baill. (under development)
| Synonyms | Kadsura chinensis Turcz. |
| Maximowiczia amurensis Rupr. | |
| Maximowiczia chinensis (Turcz.) Rupr. | |
| Maximowiczia japonica (A.Gray) K.Koch | |
| Maximowiczia sinensis Rob. | |
| Schisandra chinensis var. glabrata Nakai ex T.Mori | |
| Schisandra chinensis var. leucocarpa P.H.Huang & L.H.Zhuo | |
| Schisandra chinensis var. typica Nakai | |
| Schisandra viridicarpa Y.N.Lee | |
| Sphaerostema japonicum A.Gray | |
| Common name (en) | Chinese magnolia-vine |
| Common name (ar) | شزندرة صينية |
| Common name (de) | Chinesische Spaltkörbchen |
| Common name (fr) | Graine aux cinq saveurs |
| Common name (ja) | チョウセンゴミシ |
| Common name (ko) | 오미자 |
| Common name (ru) | Лимонник китайский |
| Common name (vi) | Ngũ vị tử bắc |
| Common name (zh) | 五味子 |
| Sample preparation | To 2.5 g of the powdered herbal drug add 10 mL of methanol. Extract at 25 °C in an ultrasonic bath for 5 min and centrifuge |
| Mobile phase | Acetic acid, ethyl acetate, toluene 2:22:46 (v/v/v) |
| Derivatization | Spray with a 100 g/L solution of sulfuric acid in methanol and heat in an oven at 120 °C for 7 min |
| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 50-mL conical flask, then add 10 mL of ethanol. Sonicate (560 W) the mixture for 1 h. Filter and transfer the filtrate to a 50-mL round bottomed flask. Evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 1 mL of ethanol |
| Mobile phase | Petroleum ether (60-80° C) , ethyl acetate, formic acid 15:5:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1.0 g of pulverized Schisandra Fruit add 10 mL of methanol, warm on a water bath for 3 minutes with shaking, cool, filter, and use the filtrate as the sample solution |
| Mobile phase | Ethyl acetate, hexane, acetic acid 10:10:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1.0 g of pulverized Schisandra Fruit add 10 mL of methanol, warm on a water bath for 3 minutes with shaking, cool, filter, and use the filtrate as the sample solution |
| Mobile phase | Ethyl acetate, hexane, acetic acid 10:10:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Weigh 1.0 g each of pulverized Schisandra Fruit, add 20 mL of dichloromethane, heat on a water bath for 30 minutes under a reflux condenser, filter and evaporate the filterate to dryness, respectively. Add 1 mL of methanol to each of the residue and use these solutions as the test solution |
| Mobile phase | Petroleum ether, ethyl formate, formic acid 15:5:1 (v/v/v) |
| Derivatization | Spray with sulfuric acid (1 volume of sulfuric acid to 2 volumes of water, and while warming on a water-bath add drop wise potassium permanganate, until a pale red color of the solution remains) and heat at 105°C |
| Sample preparation | To 1 g of the powder, add 20 mL of chloroform, heat under reflux on a water bath for 30 min, filter, span style="color:red">evaporate the filtrate to dryness. Dissolve the residue in 1 mL of chloroform |
| Mobile phase | Petroleum ether (60-80° C) , ethyl formate, formic acid 15:5:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Add 1.0 g of powdered sample to 10 mL of methanol, sonicate for 30 min, filter and use the filtrate |
| Mobile phase | Ethyl acetate, hexane, acetic acid 10:10:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Sonicate 2.5 g of Northern Schisandra Fruit Powder in 10 mL of alcohol for 10 min. Centrifuge, and use the supernatant |
| Mobile phase | Toluene, ethyl acetate, acetic acid 23:6:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 120°C for 7 min |
| System Suitability Test (SST) | According to the monograph |
| HPTLC Chromatograms |
|
| Sample preparation | 250 mg/mL of Schisandra chinensis fruit powder sonicated for 10 min and centrifuged or filtered |
| Mobile phase | Toluene, ethyl acetate, acetone, methanol 50:25:25:3 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| HPTLC Chromatograms |
|
| Sample preparation | Take 1 g of medicinal powder, add 20 ml of chloroform, reflux for about 30 min, filter, evaporate to dryness and dissolve the residue with 1 mL of chloroform |
| Mobile phase | Petroleum ether (60ºC), ethyl formate, formic acid 15:5:1 (v/v/v) |
| Derivatization | No derivatization |