Botanical Information Isatis tinctoria L. (procuring samples)
| Synonyms | Crucifera isatis E.H.L.Krause |
| Isatis alpina Vill. | |
| Isatis bannatica Link | |
| Isatis campestris Steven ex DC. | |
| Isatis canescens DC. | |
| Isatis canescens var. glabrifolia H.Lindb. | |
| Isatis ciesielskii Zapal. | |
| Isatis funebris Fed. | |
| Isatis glauca Gilib. | |
| Isatis indigotica Fortune ex Lindl. | |
| Isatis japonica Miq. | |
| Isatis kamienskii Zapal. 13: 447 (1913). | |
| Isatis koelzii Rech.f. | |
| Isatis macrocarpa B.Fedtsch. ex Nikitina | |
| Isatis maeotica DC. | |
| Isatis maritima Rupr. | |
| Isatis oblongata var. yezoensis (Ohwi) Y.L.Chang | |
| Isatis reticulata C.A.Mey. | |
| Isatis sativa (DC.) C.Knight | |
| Isatis sibirica Trautv. | |
| Isatis taurica M.Bieb. | |
| Isatis tinctoria f. glabrifolia (H.Lindb.) H.Lindb. | |
| Isatis tinctoria subsp. canescens (DC.) Arcang. | |
| Isatis tinctoria subsp. japonica (Miq.) N.Busch | |
| Isatis tinctoria subsp. koelzii (Rech.f.) Jafri | |
| Isatis tinctoria subsp. transsilvanica (Simonk.) | |
| Isatis tinctoria var. alpina (Vill.) W.D.J.Koch | |
| Isatis tinctoria var. banatica (Link) Alef. Landw. | |
| Isatis tinctoria var. campestris (Steven ex DC.) W.D.J.Koch | |
| Isatis tinctoria var. canescens (DC.) Gren. & Godr. | |
| Isatis tinctoria var. hirsuta Mérat | |
| Isatis tinctoria var. indigotica (Fortune ex Lindl.) | |
| Isatis tinctoria var. maeotica (DC.) Alef. Landw. | |
| Isatis tinctoria var. microcarpa DC. | |
| Isatis tinctoria var. sativa DC. | |
| Isatis tinctoria var. taurica (M.Bieb.) Alef. Landw. | |
| Isatis tinctoria var. vulgaris W.D.J.Koch | |
| Isatis tinctoria var. yezoensis (Ohwi) Ohwi | |
| Isatis transsilvanica Simonk. | |
| Isatis vermia Papan. | |
| Isatis villarsii Gaudin | |
| Isatis yezoensis Ohwi | |
| Common name (en) | Woad |
| Common name (de) | Färberwaid |
| Common name (es) | Hierba pastel |
| Common name (fr) | Pastel des teinturiers |
| Common name (ja) | ホソバタイセイ |
| Common name (ko) | 판람근 |
| Common name (nl) | Wede |
| Common name (ru) | Вайда красильная |
| Common name (zh) | 菘藍 |
| Sample preparation | To 0.5 g of the powder add 20 mL of chloroform, heat under reflux for 1 hour, filter and evaporate the filtrate to 1 mL as the test solution |
| Mobile phase | Cyclohexane, chloroform, acetone 5:4:2 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of ethanol (70% v/v) and sonicate for 10 min. Centrifuge and use the supernatant |
| Mobile phase | Formic acid, water, acetonitrile 2:8:30 (v/v/v) |
| Derivatization | Expose to concentrated ammonia vapour for 5 min, treat with ninhydrin solution, then heat at 120°C for 3 min |
| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 10 mL of boiling water. Sonicate (140 W) the mixture for 1 h. Centrifuge at about 1800 × g for 10 min. Transfer the supernatant to a 100-mL round-bottomed flask. Evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 1 mL of methanol and then filter |
| Mobile phase | Ammonium hydroxide solution (25%, v/v), methanol, ethyl acetate 0.5:3:7 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 0.5 g of the powder add 20 mL of dilute ethanol, ultrasonicate for 20 minutes and filter, evaporate the filtrate to dryness. Dissolve the residue with 1 mL of dilute ethanol as the test solution |
| Mobile phase | Butanol, acetic acid, water 19:5:5 (v/v/v) |
| Derivatization | Spray with ninhydrin reagent and heat the plate at 105°C until the spot appear |
| HPTLC Chromatograms |
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| Sample preparation | To 1 g of the powder add 20 mL of 80% methanol, ultrasonicate for 30 minutes and filter. Evaporate the filtrate to dryness. Dissolve the residue with 1 mL of methanol as the test solution |
| Mobile phase | Petroleum ether (60-90°C), ethyl acetate 1:1 (v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | Add 0.5 g of powdered sample to 20 mL of chloroform, heat under reflux for 1 hour, filter and evaporate the filtrate to 1 mL |
| Mobile phase | Benzene, chloroform, acetone 5:4:1 (v/v/v) |
| Derivatization | No derivatization |