Botanical Information Isatis tinctoria L. (procuring samples)

Synonyms Crucifera isatis E.H.L.Krause
Isatis alpina Vill.
Isatis bannatica Link
Isatis campestris Steven ex DC.
Isatis canescens DC.
Isatis canescens var. glabrifolia H.Lindb.
Isatis ciesielskii Zapal.
Isatis funebris Fed.
Isatis glauca Gilib.
Isatis indigotica Fortune ex Lindl.
Isatis japonica Miq.
Isatis kamienskii Zapal. 13: 447 (1913).
Isatis koelzii Rech.f.
Isatis macrocarpa B.Fedtsch. ex Nikitina
Isatis maeotica DC.
Isatis maritima Rupr.
Isatis oblongata var. yezoensis (Ohwi) Y.L.Chang
Isatis reticulata C.A.Mey.
Isatis sativa (DC.) C.Knight
Isatis sibirica Trautv.
Isatis taurica M.Bieb.
Isatis tinctoria f. glabrifolia (H.Lindb.) H.Lindb.
Isatis tinctoria subsp. canescens (DC.) Arcang.
Isatis tinctoria subsp. japonica (Miq.) N.Busch
Isatis tinctoria subsp. koelzii (Rech.f.) Jafri
Isatis tinctoria subsp. transsilvanica (Simonk.)
Isatis tinctoria var. alpina (Vill.) W.D.J.Koch
Isatis tinctoria var. banatica (Link) Alef. Landw.
Isatis tinctoria var. campestris (Steven ex DC.) W.D.J.Koch
Isatis tinctoria var. canescens (DC.) Gren. & Godr.
Isatis tinctoria var. hirsuta Mérat
Isatis tinctoria var. indigotica (Fortune ex Lindl.)
Isatis tinctoria var. maeotica (DC.) Alef. Landw.
Isatis tinctoria var. microcarpa DC.
Isatis tinctoria var. sativa DC.
Isatis tinctoria var. taurica (M.Bieb.) Alef. Landw.
Isatis tinctoria var. vulgaris W.D.J.Koch
Isatis tinctoria var. yezoensis (Ohwi) Ohwi
Isatis transsilvanica Simonk.
Isatis vermia Papan.
Isatis villarsii Gaudin
Isatis yezoensis Ohwi
Common name (en) Woad
Common name (de) Färberwaid
Common name (es) Hierba pastel
Common name (fr) Pastel des teinturiers
Common name (ja) ホソバタイセイ
Common name (ko) 판람근
Common name (nl) Wede
Common name (ru) Вайда красильная
Common name (zh) 菘藍

Sample preparation To 0.5 g of the powder add 20 mL of chloroform, heat under reflux for 1 hour, filter and evaporate the filtrate to 1 mL as the test solution
Mobile phase Cyclohexane, chloroform, acetone 5:4:2 (v/v/v)
Derivatization No derivatization
Sample preparation To 0.5 g of the powdered herbal drug add 5 mL of ethanol (70% v/v) and sonicate for 10 min. Centrifuge and use the supernatant
Mobile phase Formic acid, water, acetonitrile 2:8:30 (v/v/v)
Derivatization Expose to concentrated ammonia vapour for 5 min, treat with ninhydrin solution, then heat at 120°C for 3 min