Botanical Information Verbena officinalis L. (under development)
Synonyms | No synonym |
Common name (en) | Common vervain |
Common name (de) | Echte Eisenkraut |
Common name (es) | Verbena común |
Common name (fr) | Verveine officinale |
Common name (ja) | クマツヅラ |
Common name (ko) | 마편초 |
Common name (nl) | IJzerhard |
Common name (ru) | Вербена лекарственная |
Common name (vi) | Cỏ roi ngựa |
Common name (zh) | 马鞭草 |
Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of methanol, heat in a water-bath at 60 °C for 10 min. Cool and filter |
Mobile phase | Anhydrous formic acid, glacial acetic acid, water, ethyl acetate 11:11:27:100 (v/v/v/v) |
Derivatization | Spray with anisaldehyde solution and examine in daylight while heating at 100-105 °C for 10 min |
Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 50-mL conical flask, then add 10 mL of methanol. Sonicate (350 W) the mixture for 30 min. Filter through a 0.45-µm nylon filter |
Mobile phase | Ethyl acetate, water, formic acid, glacial acetic acid 8:2:1:1 (v/v/v/v) |
Derivatization | Spray with 1% vanillin-sulfuric acid reagent and heat the plate at 105°C until the spot appear |
Sample preparation | To 2 g of the powder, add 60 mL of 80% methanol, heat under reflux for 1 h. , filter, and evaporate the filtrate to dryness. Dissolve the residue in 2 mL of methanol as the test solution |
Mobile phase | Cyclohexane, chloroform, ethyl acetate, acetic acid 20:5:8:0.1 (v/v/v/v) |
Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spot appear |