Botanical Information Plantago ovata Forssk. (procuring samples)

Synonyms Plantago decumbens Forssk.
Plantago decumbens var. viridis Barnéoud
Plantago insularis Eastw.
Plantago ispaghul Roxb.
Plantago mauritii Sennen
Plantago ovata var. insularis (Eastw.) S.C.Meyers & Liston
Common name (en) Blond plantain
Common name (ar) الربلة
Common name (de) Indische Flohsamen
Common name (es) Ispaghul
Common name (fr) Plantain des Indes
Common name (hi) ईसबगोल
Common name (ru) Подорожник яйцевидный
Common name (th) เทียนเกล็ดหอย

Sample preparation To 50 mg of the powdered herbal drug in a thick-walled centrifuge tube add 2 mL of a 230 g/L solution of trifluoroacetic acid, and shake vigorously. Stopper the test tube and heat the mixture at 120 °C for 1 h. Centrifuge the hydrolysate, transfer the clear supernatant into a 50 mL flask, add 10 mL of water and evaporate the solution to dryness under reduced pressure. Take up the residue in 10 mL of water and evaporate again to dryness under reduced pressure. Take up the residue in 2 mL of methanol
Mobile phase Water, acetonitrile 15:85 (v/v) on Silica gel R plate
Derivatization Spray with aminohippuric acid reagent (dissolve 3 g of phthalic acid and 0.3 g of aminohippuric acid in ethanol (96%) and dilute to 100 mL with ethanol (96%)) and heat at 120 °C for 5 min