Botanical Information Wolfiporia extensa (Peck) Ginns (under development)
| Synonyms | Poria cocos F.A.Wolf |
| Common name (en) | Indian bread |
| Common name (fr) | Pachyme |
| Common name (ja) | マツホド |
| Common name (ko) | 복령 |
| Common name (vi) | Phục linh |
| Common name (zh) | 茯苓 |
| Sample preparation | To 1 g of the powdered herbal drug add a mixture of 2 mL of ethyl acetate and 3 mL of methanol. Sonicate for 10 min, centrifuge and use the supernatant |
| Mobile phase | Acetic acid, 2-propanol, cyclohexane 10:10:80 (v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | To 1 g of the powder add 5 mL of ethyl acetate, ultrasonicate for 10 minutes and use the supernatant as the test solution |
| Mobile phase | Toluene, ethyl acetate, formic acid 20:5:0.5 (v/v/v) |
| Derivatization | Spray with vanillin (to 90 mL of methanol and carefully add, dropwise, 10 mL of concentrated sulfuric acid, cool, then dissolve 2 g of vanillin in the prepared 10% sulfuric acid in methanol), heat at 105°C for 3 min |
| HPTLC Chromatograms |
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| Sample preparation | To 1 g of the powder add 50 mL of ether, ultasonicate for 10 minutes and filter, evaporate the filtrate to dryness and dissolve the residue in 1 mL of methanol as test solution |
| Mobile phase | Toluene, ethyl acetate, formic acid 20:5:0.5 (v/v/v) |
| Derivatization | Spray with 2% vanillin in sulfuric acid and ethanol 4:1 (v/v), and heat at 105°C until the spots appear |
| HPTLC Chromatograms |
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| Sample preparation | Add 1.0 g of powdered sample to 10 mL of methanol, shake for 5 minutes, stand, filter, and use the filtrate |
| Mobile phase | Toluene, ethyl acetate 1:1 (v/v) |
| Derivatization | Spray with a solution of anisaldehyde in sulfuric acid and heat at 105°C until the spots appear |
| Sample preparation | Take 1 g of medicinal powder, add 20 ml of ether, sonicate for 10 min, filter, evaporate to dryness and dissolve the residue with 1 mL of methanol |
| Mobile phase | Toluene, ethyl acetate, formic acid 20:5:0.5 (v/v/v) |
| Derivatization | Spray with 2% vanillin reagent in sulfuric acid, ethanol 4:1 and heat the plate at 105°C until the spot appear |