Botanical Information Melissa officinalis L. (under development)

Synonyms Faucibarba officinalis (L.) Dulac
Mutelia officinalis (L.) Gren. ex Mutel
Thymus melissa E.H.L.Krause
Common name (en) Lemon balm
Common name (af) Suurlemoenbalsem
Common name (de) Zitronenmelisse
Common name (fr) Mélisse officinale
Common name (it) Melissa vera
Common name (ja) レモンバーム
Common name (ko) 레몬밤
Common name (nl) Citroenmelisse
Common name (pt) Erva-cidreira
Common name (ru) Мелисса лекарственная
Common name (th) สะระแหน่
Common name (vi) Tía tô đất
Common name (zh) 蜜蜂花

Sample preparation Place 2.0 g of the powdered herbal drug in a 250 mL round-bottomed flask and add 100 mL of water Distil for 1 h using the apparatus for the determination of essential oils in herbal drugs and 0.5 mL of xylene in the graduated tube. After distillation transfer the organic phase to a 1 mL volumetric flask, rinsing the graduated tube of the apparatus with the aid of a small portion of xylene, and dilute to 1.0 mL with the same solvent
Mobile phase Ethyl acetate, hexane 10:90 (v/v)
Derivatization Spray with anisaldehyde solution and heat at 100-105°C for 10-15 min