HPTLC ATLAS

 

Botanical Information Cannabis sativa L. (under development)

Synonyms Cannabis americana Pharm. ex Wehmer
Cannabis chinensis Delile
Cannabis erratica Siev.
Cannabis foetens Gilib.
Cannabis generalis E.H.L.Krause
Cannabis gigantea Crevost
Cannabis indica Lam.
Cannabis indica f. afghanica (Vavilov) Vavilov
Cannabis indica var. kafiristanica Vavilov
Cannabis intersita Soják
Cannabis kafiristanica (Vavilov)
Cannabis lupulus Scop.
Cannabis macrosperma Stokes
Cannabis ruderalis Janisch.
Cannabis sativa f. afghanica Vavilov
Cannabis sativa f. chinensis (Delile) A.DC.
Cannabis sativa f. pedemontana A.DC.
Cannabis sativa f. vulgaris (Alef.)
Cannabis sativa subsp. indica (Lam.)
Cannabis intersita (Soják) Soják
Cannabis sativa var. gigantea (Delile ex Vilm.) Alef.
Cannabis sativa var. indica (Lam.) Wehmer
Cannabis sativa var. kafiristanica (Vavilov)
Cannabis sativa var. kif A.DC.
Cannabis sativa var. macrosperma (Stokes) Asch. & Graebn.
Cannabis sativa var. monoica Holuby
Cannabis sativa var. praecox Serebr.
Cannabis sativa var. ruderalis (Janisch.) S.Z.Liou
Cannabis sativa var. ruderalis Janisch.
Cannabis sativa var. spontanea Vavilov
Cannabis sativa var. vulgaris Alef.
Polygonum viridiflorum Poir.
Common name (en) Cannabis
Common name (de) Hanf
Common name (es) Marihuana
Common name (fr) Chanvre
Common name (hi) गाँजे का पौधा
Common name (it) Canapa sativa
Common name (ko)
Common name (nl) Hennep
Common name (ru) Конопля посевная
Common name (th) กัญชง
Common name (zh) 大麻

Sample preparation 0.1 g of the powdered herbal drug is mixed with 5 mL of methanol, sonicated for 10 min and centrifuged. The supernatant is used as test solution
Mobile phase Methanol, water, acetic acid 70:15:15 (v/v/v) on HPTLC Si RP-18 F254
Derivatization Spray with vanillin reagent (1 g of Vanillin is dissolved in 100 mL of ethanol 96% and 2 mL of sulfuric acid are carefully added to this solution), and heat the plate at 100°C for 3 min.
Sample preparation Macerate 1.0 g of powdered drug in 10 mL of methanol for 6 hours. Filter and evaporate to dryness. Dissolve the residue in 10 mL of toluene
Mobile phase n-hexane, diethyl ether 8:2 (v/v)
Derivatization Spray with anisaldehyde solution and heat at 110°C until the colored bands appear

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