Botanical Information Magnolia officinalis Rehder & E.H.Wilson (under development)
| Synonyms | Houpoea officinalis (Rehder & E.H.Wilson) N.H.Xia & C.Y.Wu |
| Common name (en) | Houpu magnolia |
| Common name (ar) | ماغنوليا طبية |
| Common name (ru) | Магнолия лекарственная |
| Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of methanol, sonicate for 5 min, centrifuge, and use the supernatant. Filter through a membrane filter (nominal pore size 0.45 µm) if necessary |
| Mobile phase | Methanol, ethyl acetate, toluene 4:8:120 (v/v/v) |
| Derivatization | Treat with vanillin reagent, heat at 100-105 °C for 5-7 min and examine |
| Sample preparation | To 0.5 g of the powdered herbal drug add 3.0 mL of methanol. Sonicate for 15 min and centrifuge for 15 min. Transfer the supernatant to a 5 mL flask. Add 2 mL of methanol to the residue, sonicate for 15 min and centrifuge. Transfer the supernatant into the same 5 mL flask. Dilute the combined supernatants to 5.0 mL with methanol. Filter through a membrane filter (nominal pore size 0.45 µm) if necessary |
| Mobile phase | Methanol, ethyl acetate, toluene 1:5:30 (v/v/v) |
| Derivatization | Treat with vanillin reagent, heat at 100-105 °C for 5-7 min and examine |
| Sample preparation | Weigh 0.5 g of the powdered sample and place it in a 15-mL centrifuge tube, then add 10 mL of methanol. Sonicate (140 W) the mixture for 30 min. Centrifuge at about 2800 × g for 10 min. Filter through a 0.45-µm nylon filter |
| Mobile phase | Cyclohexane, ethyl acetate 7:3 (v/v) |
| Derivatization | Spray with 5% vanillin-sulfuric acid reagent and heat the plate at 105°C until the spot appear (3-5 min) |
| Sample preparation | To 1 g of the powder add 8 mL of methanol and shake for 30 min, filter, and use the filtrate as the test solution |
| Mobile phase | Cyclohexane, dichloromethane, ethyl acetate, ammonia 5:2:4:0.5 (v/v/v/v) |
| Derivatization | Spray with 1% vanillin-sulfuric acid reagent and heat the plate at 100°C until the spot appear |