Botanical Information Plantago asiatica L. (procuring samples)

Synonyms Plantago alata Nakai Saishu-to Kuan-to Shokubutsu Hokoku-sho
Plantago asiatica f. folioscopa (T.Itô) Honda
Plantago asiatica f. paniculata Hara
Plantago asiatica f. polystachya Nakai
Plantago asiatica f. rosea Makino ex Nakai
Plantago asiatica f. yakusimensis (Masam.)
Plantago asiatica var. brevior Pilg. Notizbl.
Plantago asiatica var. coreana (H.Lév.)
Plantago asiatica var. densiuscula Pilg.
Plantago asiatica var. laxa Pilg.
Plantago asiatica var. lobulata Pilg.
Plantago asiatica var. yezoensis (Pilg.) H.Hara
Plantago coreana H.Lév. Repert.
Plantago formosana Tateishi & Masam.
Plantago hostifolia Nakai & Kitag.
Plantago major f. asiatica (L.) Regel
Plantago major var. asiatica (L.) Decne.
Plantago major var. folioscopa T.Itô
Plantago taquetii H.Lév. Repert.
Plantago yakusimensis Masam.
Plantago yezoensis Pilg.
Common name (ja) オオバコ
Common name (ko) 질경이
Common name (vi) Xa tiền
Common name (zh) 车前

Sample preparation Weigh 2.5 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 10 mL of methanol (60%). Sonicate (350 W) the mixture for 15 min. Centrifuge at about 4000 × g for 5 min. Filter through a 0.45-µm nylon filter
Mobile phase Ethyl acetate, ethanol, formic acid, water 18:3:1.5:1 (v/v/v/v)
Derivatization No derivatization
Sample preparation Weigh 1.0 g of the powdered sample and place it in a 50-mL conical flask, then add 10 mL of methanol. Sonicate (200 W) the mixture for 15 min. Filter the mixture
Mobile phase Ethyl acetate, water, formic acid, glacial acetic acid 8:2:1:1 (v/v/v/v)
Derivatization Spray with 1% vanillin sulfuric acid reagent in ethanol, and heat at 105°C until the sppots appear