HPTLC ATLAS

 

Botanical Information Dioscorea collettii var. hypoglauca (Palib.) S.J.Pei & C.T.Ting (procuring samples)

Synonyms Dioscorea hypoglauca Palib.
Dioscorea kaoi T.S.Liu & T.C.Huang
Dioscorea morsei Prain & Burkill
Dioscorea undulata R.Knuth
Common name (en) Hypoglaucous Collett yam

Sample preparation To 0.5 g of the powder, add 25 mL of methanol, ultrasonicate for 30 min and filter. Evaporate the filtrate to dryness and dissolve the residue in 2 mL of methanol as the test solution
Mobile phase Chloroform, methanol, water 13:7:2 (v/v/v), keeping below 10°C
Derivatization Spray with 5% sulfuric acid reagent and heat the plate at 105°C until the spot appear

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