Botanical Information Dioscorea collettii var. hypoglauca (Palib.) S.J.Pei & C.T.Ting (procuring samples)
Synonyms | Dioscorea hypoglauca Palib. |
Dioscorea kaoi T.S.Liu & T.C.Huang | |
Dioscorea morsei Prain & Burkill | |
Dioscorea undulata R.Knuth | |
Common name (en) | Hypoglaucous Collett yam |
Sample preparation | To 0.5 g of the powder, add 25 mL of methanol, ultrasonicate for 30 min and filter. Evaporate the filtrate to dryness and dissolve the residue in 2 mL of methanol as the test solution |
Mobile phase | Chloroform, methanol, water 13:7:2 (v/v/v), keeping below 10°C |
Derivatization | Spray with 5% sulfuric acid reagent and heat the plate at 105°C until the spot appear |
Sample preparation | Add 0.5 g of powdered sample to 25 mL of methanol, ultrasonicate for 30 min, cool, filter, evaporate the filtrate to dryness, and make up to 2 mL |
Mobile phase | Chloroform, methanol, water 13:7:2 (v/v/v), keeping below 10°C |
Derivatization | Spray with 10% sulfuric acid in ethanol and heat until the spots appear |