Botanical Information Plantago depressa Willd. (procuring samples)

Synonyms Plantago asiatica var. decumbens Turcz.
Plantago depressa f. glaberrima Kom.
Plantago depressa f. magnibracteata Tosh.Tanaka & T.K.Zheng
Plantago depressa f. minor Kom.
Plantago depressa var. eudepressa Ganesch.
Plantago paludosa Turcz. ex Ledeb.
Plantago sibirica Georgi Beschr.
Plantago sibirica Poir.
Plantago tibetica Hook.f. & Thomson

Sample preparation Weigh 2.5 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 10 mL of methanol (60%). Sonicate (350 W) the mixture for 15 min. Centrifuge at about 4000 × g for 5 min. Filter through a 0.45-µm nylon filter
Mobile phase Ethyl acetate, ethanol, formic acid, water 18:3:1.5:1 (v/v/v/v)
Derivatization No derivatization
Sample preparation Add 2.0 g of powdered sample to 10 mL of ethanol, heat under reflux for 30 min, cool, filter and make up the filtrate to 10 mL
Mobile phase n-butanol, acetic acid, water 7:1:2 (v/v/v)
Derivatization Spray with 2,4-dinitrophenylhydrazine and heat at 105°C for 2 min