Botanical Information Nigella sativa L. (under development)
| Synonyms | Nigella cretica Mill. |
| Nigella glandulifera Freyn & Sint. | |
| Nigella indica Roxb. | |
| Nigella sativa var. brachyloba Boiss. | |
| Nigella sativa var. citrina DC. | |
| Nigella sativa var. cretica (Mill.) DC. | |
| Nigella sativa var. hispidula Boiss. | |
| Nigella sativa var. indica (Roxb.) DC. | |
| Nigella truncata Viv. | |
| Solanum dulcamara var. plenum G.Don | |
| Common name (en) | Black cumin |
| Common name (de) | Echte Schwarzkümmel |
| Common name (fr) | Nigelle cultivée |
| Common name (it) | Cumino nero |
| Common name (ko) | 흑쿠민 |
| Common name (nl) | Zwarte komijn |
| Common name (ru) | Чернушка посевная |
| Sample preparation | Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge the solution and use the supernatant as test solution |
| Mobile phase | Toluene, cyclohexane, acetone 8:2:0.5 (v/v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under UV 254 nm, prior to derivatization
|
| Sample preparation | Weigh accurately 1 g of the powder to a soxhlet’s extractor; add a quantity of petroleum ether (60-90°C), heat under efflux for 2 hours. Discard the petroleum ether extract, expel solvent from the residue and add a quantity of methanol, heat under reflux for 4 hours, and evaporate to dryness. Dissolve the residue in 15 mL of water saturated with n-butanol, transfer to a separator funnel, and extract by shaking with three 20 mL quantities of n-butanol saturated with water. Combine the n-butanol solutions and evaporate to dryness, dissolve the residue in a mixture of 20 mL of methanol and 2 mL of hydrochloric acid, heat under reflux for 4 hours, cool, add 10 mL of water, mix well, and extract with three 20 mL quantities of chloroform. Combine the chloroform and evaporate to dryness, dissolve the residue in methanol, transfer to a 10 mL volumetric flask, add methanol to volume, mix well and filter. Use the successive filtrate as test solution |
| Mobile phase | Cyclohexane, ethyl acetate, acetic acid 6:4:0.25 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear |
| Sample preparation | Shake 3 g of the sample in 10 mL of methanol for 2 minutes and filter. Use the filtrate as test solution |
| Mobile phase | Toluene, ethyl acetate 9:1 (v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 105°C for 15 min |
| Sample preparation | Dissolve 0.2 g of black cumin seed oil with 10 mL of acetone |
| Mobile phase | Toluene, cyclohexane, acetone 8:2:0.5 (v/v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under UV 254 nm, prior to derivatization
|
| Sample preparation | Dissolve 25 µL of black cumin seed oil in 3 mL of dichloromethane |
| Mobile phase | On HPTLC-RP-18 F254s (Merck), pre-develop the plate with dichloromethane to the upper edge. Dry the plate at 120 °C for 20 min. Develop with dichloromethane, acetic acid, acetone 20:40:50 (v/v/v) until 80 mm from lower edge |
| Derivatization | Spray with phosphomolybdic acid reagent (dissolve 2 g of phosphomolybdic acid in 20 mL of water, add gently 30 mL of sulfuric acid, mix well), and heat at 120 °C for 3 min |
| System Suitability Test (SST) | According to the monograph |