HPTLC ATLAS

 

Botanical Information Nigella sativa L. (under development)

Synonyms Nigella cretica Mill.
Nigella glandulifera Freyn & Sint.
Nigella indica Roxb.
Nigella sativa var. brachyloba Boiss.
Nigella sativa var. citrina DC.
Nigella sativa var. cretica (Mill.) DC.
Nigella sativa var. hispidula Boiss.
Nigella sativa var. indica (Roxb.) DC.
Nigella truncata Viv.
Solanum dulcamara var. plenum G.Don
Common name (en) Black cumin
Common name (de) Echte Schwarzkümmel
Common name (fr) Nigelle cultivée
Common name (it) Cumino nero
Common name (ko) 흑쿠민
Common name (nl) Zwarte komijn
Common name (ru) Чернушка посевная

Sample preparation Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge the solution and use the supernatant as test solution
Mobile phase Toluene, cyclohexane, acetone 8:2:0.5 (v/v/v)
Derivatization Spray with anisaldehyde solution and heat at 100°C for 3 min
System Suitability Test (SST) Under development
Specific marker(s) Under UV 254 nm, prior to derivatization
  • carvacrol: quenching zone at RF ~ 0.42
  • thymoquinone: quenching zone at RF ~ 0.63
Sample preparation Dissolve 0.2 g of black cumin seed oil with 10 mL of acetone
Mobile phase Toluene, cyclohexane, acetone 8:2:0.5 (v/v/v)
Derivatization Spray with anisaldehyde solution and heat at 100°C for 3 min
System Suitability Test (SST) Under development
Specific marker(s) Under UV 254 nm, prior to derivatization
  • carvacrol: quenching zone at RF ~ 0.42
  • thymoquinone: quenching zone at RF ~ 0.63

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