Botanical Information Periploca sepium Bunge (under development)
| Synonyms | No synonym |
| Common name (de) | Chinesische Baumschlinge |
| Common name (fr) | Périploque de Chine |
| Common name (zh) | 香加皮 |
| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 25-mL conical flask, then add 10 mL of ethanol. Sonicate (150 W) the mixture for 30 min. Filter the mixture |
| Mobile phase | Petroleum ether (60-80°C) , ethyl acetate 4:1 (v/v) |
| Derivatization | Spray with 2,4-dinitrophenylhydrazine reagent (0.1 g of 2,4-dinitrophenylhydrazine dissolved in 100 mL of a mixture of ethanol and hydrochloric acid 99:1 (v/v)) |
| Sample preparation | To 0.3 g of the powdered herbal drug add 3 mL of methanol, heat in a water-bath at 60 °C for 1 min and filter |
| Mobile phase | Petroleum ether (60-80ºC), ethyl acetate 4:1 (v/v) |
| Derivatization | Treat the plate with a mixture of 5 mL freshly prepared 2% methanolic 3,5-dinitrobenzoic acid and 5 mL of 5-7% methanolic sodium hydroxide solution |
| Sample preparation | To 2 g of the powder add 30 mL of methanol, heat under reflux for 1 hour, and filter. Evaporate the filtrate to dryness, and dissolve the residue in 2 mL of methanol as the test solution |
| Mobile phase | Petroleum ether (60-90°C) , ethyl acetate, acetic acid 20:3:0.5 (v/v/v) |
| Derivatization | Spray with 2,4-dinitrophenylhydrazine reagent |