Botanical Information Leonurus japonicus Houtt. (under development)

Synonyms Leonurus altissimus Bunge ex Benth.
Leonurus artemisia (Lour.) S.Y.Hu
Leonurus artemisia var. albiflorus (Migo) S.Y.Hu
Leonurus cuneifolius Raf.
Leonurus heterophyllus Sweet
Leonurus heterophyllus f. leucanthus C.Y.Wu & H.W.Li
Leonurus intermedius Didr.
Leonurus japonicus f. albiflorus (Migo) Y.C.Zhu
Leonurus japonicus f. niveus (A.I.Baranov & Skvortsov) H.Hara
Leonurus mexicanus Sessé & Moc.
Leonurus sibiricus f. niveus A.I.Baranov & Skvortsov
Leonurus sibiricus var. albiflorus Migo
Stachys artemisia Lour.
Common name (en) Oriental motherwort
Common name (ja) メハジキ
Common name (ko) 익모초
Common name (th) เอียะบ้อเช่า
Common name (vi) Ích mẫu
Common name (zh) 益母草

Sample preparation Weigh 2.0 g of the powdered sample and place it in a cellulose extraction thimble. Place the cellulose extraction thimble in soxhlet extractor. Add 300 mL of ethyl acetate to a 500-mL round bottomed flask. Perform the soxhlet extraction for about 12 h. Collect and air dry the residue. Transfer the residue to a 50-mL conical flask. Add 25 mL of absolute ethanol and 150 μL of 0.1 M hydrochloric acid. Sonicate (220 W) the mixture for 30 min. Filter and evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 0.5 mL of absolute ethanol
Mobile phase n-butanol, hydrochloric acid, ethyl acetate 8:2:0.5 (v/v/v)
Derivatization Spray with the reagent (Dissolve 0.85 g of bismuth subnitrate in 50 mL of acetic acid (20%, v/v) and then add 20 mL of potassium iodide solution (40%, w/v) to obtain potassium iodobismuthate solution. Transfer 1 mL of potassium iodobismuthate solution to a 10-mL volumetric flask. Add 2 mL of 0.6 M hydrochloric acid and make up to the mark with water)