Botanical Information Prunella vulgaris L. (procuring samples)
| Synonyms | Prunella vulgaris subsp. parviflora Ehrh. |
| Common name (en) | Common self-heal |
| Common name (de) | Kleine Braunelle |
| Common name (es) | Consuelda menor |
| Common name (fr) | Brunelle commune |
| Common name (ja) | ウツボグサ |
| Common name (ko) | 꿀풀 |
| Common name (nl) | Gewone brunel |
| Common name (ru) | Черноголовка обыкновенная |
| Common name (th) | แฮ่โกวเฉ่า |
| Common name (zh) | 夏枯草 |
| Sample preparation | Weigh 2.5 g of the powdered sample and place it in a 100-mL conical flask, then add 30 mL of ethanol (70%). Sonicate (90 W) the mixture for 30 min. Filter and evaporate the filtrate to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 5 mL of ethanol. Filter through a 0.45-µm PTFE filter |
| Mobile phase | Cyclohexane, ethyl acetate, isopropanol, formic acid 7:3:2:0.3 v/v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear |
| Sample preparation | To 2.5 g of the powder, add 30 mL of 70% ethanol, ultrasonicate for 30 min, filter and evaporate the filtrate to dryness. Dissolve the residue in 5 mL of ethanol to produce a solution containing 0.1 mg per mL |
| Mobile phase | Cyclohexane, ethyl acetate, isopropanol, formic acid 15:3:3.5:0.5 (v/v/v/v) |
| Derivatization | No derivatization |