Botanical Information Prunella vulgaris L. (procuring samples)

Synonyms Prunella vulgaris subsp. parviflora Ehrh.
Common name (en) Common self-heal
Common name (de) Kleine Braunelle
Common name (es) Consuelda menor
Common name (fr) Brunelle commune
Common name (ja) ウツボグサ
Common name (ko) 꿀풀
Common name (nl) Gewone brunel
Common name (ru) Черноголовка обыкновенная
Common name (th) แฮ่โกวเฉ่า
Common name (zh) 夏枯草

Sample preparation Weigh 2.5 g of the powdered sample and place it in a 100-mL conical flask, then add 30 mL of ethanol (70%). Sonicate (90 W) the mixture for 30 min. Filter and evaporate the filtrate to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 5 mL of ethanol. Filter through a 0.45-µm PTFE filter
Mobile phase Cyclohexane, ethyl acetate, isopropanol, formic acid 7:3:2:0.3 v/v/v/v)
Derivatization Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear