Botanical Information Coptis deltoidea C.Y.Cheng & P.K.Hsiao (under development)
Synonyms | No synonym |
Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of methanol. Sonicate for 15 min. Centrifuge the solution and use the supernatant |
Mobile phase | Water, methanol, 2-propanol, ethyl acetate, toluene 1:5:5:10:20(v/v/v/v/v). For saturation, add mobile phase to one trough and concentrated ammonia to the other |
Derivatization | No derivatization |
Sample preparation | Weigh 0.5 g of the powdered sample and put into a 100-mL conical flask, then add 20 mL of methanol. Sonicate (560 W) the mixture for 30 min. Filter and evaporate the filtrate to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 4 mL of methanol |
Mobile phase | Toluene, ethyl acetate, methanol, 2-propanol, ammonia solution 12:6:3: 3:1 (v/v/v/v/v) |
Derivatization | No derivatization |
Sample preparation | To 0.5 g of pulverized Coptis Rhizome add 20 mL of methanol, shake for 2 minutes, filter, and use the filtrate as the sample solution |
Mobile phase | 1-butanol, water, acetic acid 7:2:1 (v/v/v) |
Derivatization | No derivatization |
Sample preparation | Weigh 0.5 g of pulverized Coptis Rhizome, add 20 mL of methanol, shake for 2 minutes, filter and use the filtrate as the test solution |
Mobile phase | n-butanol, acetic acid, water 7:1:2 (v/v/v) |
Derivatization | No derivatization |
Sample preparation | To 0.25 g of the powder add25 mL of methanol, ultrasonicate for 30 min, filter and use the filtrate as the test solution |
Mobile phase | Cyclohexane, ethyl acetate, isopropanol, methanol, water, triethylamine 3:3.5:1:1.5:0.5:1 (v/v/v/v/v/v) in chamber saturated with ammonia |
Derivatization | No derivatization |