HPTLC ATLAS

 

Botanical Information Dioscorea panthaica Prain & Burkill (procuring samples)

Synonyms Dioscorea biserialis Prain & Burkill
Common name (en) Panthaica yam

Sample preparation To 0.5 g of the powder, add 25 mL of methanol, ultrasonicate for 30 min and filter. Evaporate the filtrate to dryness and dissolve the residue in 0.5 mL of methanol as the test solution
Mobile phase Chloroform, methanol, water 75:35:4 (v/v/v)
Derivatization Spray with 10% sulfuric acid reagent and heat the plate at 105°C until the spots appear

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