Botanical Information Salvia miltiorrhiza Bunge (under development)
| Synonyms | No synonym |
| Common name (en) | red sage |
| Common name (de) | Rotwurzel-Salbei |
| Common name (ja) | 丹参 |
| Common name (ko) | 단삼 |
| Common name (th) | ตังเซียม |
| Common name (vi) | Đan sâm |
| Common name (zh) | 丹参 |
| Sample preparation | To 1 g of the powdered herbal drug add 40 mL of methanol. Sonicate for 15 min. Filter. Evaporate the filtrate to 1 mL |
| Mobile phase | Methanol, anhydrous formic acid, toluene, methylene chloride, ethyl acetate 5:20:20:30:40 (v/v/v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Weigh 1.0 g of the powdered sample and put into a 50-mL centrifugal tube, then add 10 mL of diethyl ether. Close the cap of the tube, shake occasionally for 1 h and centrifuge at about 1200 x g for 10 min. Transfer the supernatant to a new tube and evaporate to dryness on a warm water bath maintained at a temperature below 50 ?C. Dissolve the residue in 0.5 mL of methanol |
| Mobile phase | Petroleum ether (60–80 ?C), ethyl acetate, cyclohexane 5:3:2 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of ethyl acetate. Sonicate for 10 min and centrifuge |
| Mobile phase | Toluene, ethyl acetate, acetic acid 95:5:1 (v/v/v) on HPTLC Si 60 F254 caffeine impregnated |
| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | To 1.0 g of pulverized Salvia Miltiorrhiza Root add 10 mL of diethyl ether, allow to stand for 10 minutes with occasional shaking, and filter. Evaporate the filtrate on a water bath to dryness, dissolve the residue in 1 mL of ethyl acetate, and use this solution as the sample solution |
| Mobile phase | n-hexane, ethyl acetate 33:1 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | Weigh 2 g of pulverized Salvia Miltiorrhiza Root, add 10 mL of methanol, sonicate for 1 hour, filter and use the fitrate as the test solution |
| Mobile phase | n-hexane, ethyl acetate 4:1 (v/v) |
| Derivatization | Spray with sulfuric acid (1 volume of sulfuric acid to 2 volumes of water, and while warming on a water-bath add drop wise potassium permanganate, until a pale red color of the solution remains) and heat |
| Sample preparation | to 1 g of the powder add 5 mL of ethanol, ultrasonicate for 15 min, centrifuge, and use the supernatant as test solution |
| Mobile phase |
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| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | Add 1.0 g of powdered sample to 5 mL of ethyl ether, shake for 1 hour, stand and filter, evaporate the filtrate to dryness. Add 1 mL of ethyl acetate to the residue |
| Mobile phase | Petroleum ether (30-60°C), ethyl acetate 4:1 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | 1.0 g of Chinese Salvia, finely powdered, in 5.0 mL of alcohol. Sonicate for 15 min, centrifuge, and use the supernatant |
| Mobile phase |
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| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| System Suitability Test (SST) | According to the monograph |
| HPTLC Chromatograms |
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| Sample preparation | About 1.0 g of Chinese Salvia, finely powdered, in 5.0 mL of alcohol. Sonicate for 15 min, centrifuge, and use the supernatant |
| Mobile phase | Toluene, dichloromethane, ethyl acetate, methanol, formic acid 4:6:8:1:4 (v/v/v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | Take 1 g of powder, add 5 mL of ether, stand for 1 hour and filter. Evaorate to dryness and dissolve theresidue with 1 mL of ethyl acetate |
| Mobile phase | Petroleum ether (60-90ºC), ethyl acetate 4:1 (v/v) |
| Derivatization | No derivatization |