Botanical Information Centella asiatica (L.) Urb
| Synonyms | Centella asiatica var. crista Makino |
| Centella boninensis Nakai ex Tuyama | |
| Centella coriacea Nannf. | |
| Centella glochidiata (Benth.) Drude | |
| Centella hirtella Nannf. | |
| Centella tussilaginifolia (Baker) Domin | |
| Centella ulugurensis (Engl.) Domin | |
| Centella uniflora (Colenso) Nannf. | |
| Chondrocarpus asiaticus Nutt. | |
| Chondrocarpus triflorus Nutt. | |
| Glyceria asiatica Nutt. | |
| Glyceria triflora Nutt. | |
| Hydrocotyle abbreviata A.Rich. | |
| Hydrocotyle abyssinica Gand. | |
| Hydrocotyle artensis Montrouz. | |
| Hydrocotyle asiatica L. | |
| Hydrocotyle asiatica var. monantha F.Muell. | |
| Hydrocotyle brasiliensis Scheidw. | |
| Hydrocotyle brevipedata St.-Lag. | |
| Hydrocotyle brevipes DC. | |
| Hydrocotyle dentata A.Rich. | |
| Hydrocotyle ficarifolia Stokes | |
| Hydrocotyle ficarioides Lam. | |
| Hydrocotyle glochidiata Benth. | |
| Hydrocotyle hebecarpa DC. | |
| Hydrocotyle inaequipes DC. | |
| Hydrocotyle indivisa Banks & Sol. ex Hook.f. | |
| Hydrocotyle lunata Lam. | |
| Hydrocotyle lurida Hance | |
| Hydrocotyle nummularioides A.Rich. | |
| Hydrocotyle pallida DC. | |
| Hydrocotyle reniformis Walter | |
| Hydrocotyle sarmentosa Salisb. | |
| Hydrocotyle sylvicola Cordem. | |
| Hydrocotyle thunbergiana Spreng. | |
| Hydrocotyle tussilaginifolia Baker | |
| Hydrocotyle ulugurensis Engl. | |
| Hydrocotyle uniflora Colenso | |
| Hydrocotyle wightiana Wall. | |
| Neosciadium glochidiatum (Benth.) Domin | |
| Trisanthus cochinchinensis Lour. | |
| Common name (en) | Gotu kola |
| Common name (de) | Indischer Wassernabel |
| Common name (hi) | मण्डूकपर्णी |
| Common name (id) | Pegagan |
| Common name (ja) | ツボクサ |
| Common name (ko) | 병풀 |
| Common name (ru) | Центелла азиатская |
| Common name (th) | บัวบก |
| Common name (vi) | Rau má |
| Common name (zh) | 崩大碗 |
| Sample preparation | Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 min, then centrifuge the solution and use the supernatants as test solutions |
| Mobile phase | Dichloromethane, methanol, water 14:6:1 (v/v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| Sample preparation | Alcoholic extract |
| Mobile phase | Chloroform, methanol 8:2 (v/v) |
| Derivatization | Exposure to iodine |
| HPTLC Chromatograms |
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| Sample preparation | Alcoholic extract |
| Mobile phase | Chloroform, methanol 8:2 (v/v) |
| Derivatization | Derivatize with vanillin sulfuric acid reagent, then heat the plate at 105°C for 10 min |
| HPTLC Chromatograms |
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| Sample preparation | To 0.5 g of the powdered herbal drug add 5 mL of ethanol 96%, sonicate for 10 min and centrifuge. Use the supernatant |
| Mobile phase | Water, methanol, dichloromethane 1:6:14 (v/v/v) |
| Derivatization | Derivatize with 10% sulfuric acid reagent, then heat the plate at 120°C for 3 min |
| HPTLC Chromatograms |
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| Sample preparation | Weigh 0.5 g of the powdered sample and place it in a 25-mL conical flask, then add 10 mL of ethanol (70%). Sonicate (150 W) the mixture for 30 min. Filter the mixture |
| Mobile phase | Ethyl acetate, glacial acetic acid, water 8:2.6:2.5 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid reagent in ethanol, then dry in air |
| Sample preparation | Reflux 2 g of coarsely powdered substance under examination with 50 mL methanol for 15 minutes, cool and filter. Reflux the residue further with 2 x 50 mL of methanol, cool and filter. Combine all the filtrates and concentrate under vacuum to 10 mL |
| Mobile phase | Chloroform, acetic acid, methanol, water 60:32:12:8 (v/v/v/v) |
| Derivatization | Derivatize with anisaldehyde sulfuric acid reagent, then heat the plate at 100°C for 5-10 min |
| HPTLC Chromatograms |
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| Sample preparation | Weigh about 5.0 g of C. asiatica dried herb powder in a 50 mL round bottom flask and add 50 mL ethanol into the flask. Reflux the sample for 1 h and allow to cool. Transfer 500 µL of the extract and add with 500 µL of ethanol. Filter the mixture and use the filtrate as the test solution |
| Mobile phase | Ethyl acetate, acetic acid, methanol, water 100:25:10:5 (v/v/v/v) |
| Derivatization | Derivatize with vanillin sulphuric acid reagent, then heat the plate at 110°C for 5-10 min |
| HPTLC Chromatograms |
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| Sample preparation | Add 10 mL of methanol to 0.5 g of the herbal drug. Sonicate for 10 min and centrifuge. Use the supernatant |
| Mobile phase | Dichloromethane, methanol, water 14:6:1 (v/v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3-5 min |
| System Suitability Test (SST) | Under white light, after derivatization
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| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| Sample preparation | Reflux 1 g of the powdered sample with 20 mL of ethanol for 10 minutes and filtering. Evaporate the filtrate under reduced pressure at 40°C until dryness, and dissolve the residue in 4 mL of ethanol |
| Mobile phase | Chloroform, methanol 7:1 (v/v) |
| Derivatization | Derivatize with anisaldehyde sulfuric acid reagent, then heat the plate at 105°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | About 0.5 g of Powdered Centella asiatica in 5 mL of methanol. Sonicate for 10 min, centrifuge, and use the supernatant |
| Mobile phase | Dichloromethane, methanol, water 14:6:1 (v/v/v) |
| Derivatization | Derivatize with 10% sulfuric acid reagent, then heat the plate at 120°C for 3 min |
| System Suitability Test (SST) | According to the monograph |
| HPTLC Chromatograms |
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| Sample preparation | 1 g of powder, add 50 mL of ether, 10 min ultrasound, filter, evaporate the filtrate, dissolve with 1 mL methanol |
| Mobile phase | Toluene, ethyl acetate, formic acid 20:5:0.5 (v/v/v) |
| Derivatization | Derivatize with sulfuric acid reagent, then heat the plate at 105°C until the spot appear |
| HPTLC Chromatograms |
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