HPTLC Association | HPTLC ATLAS

Synonyms	Anisocalyx limnanthiflorus (L.) Hance
	Bacopa micromonnieria (Griseb.) B. L. Rob.
	Bramia indica Lam.
	Bramia micromonnieria (Griseb.) Pennell
	Bramia monnieri (L.) Drake
	Bramia monnieri (L.) Pennell
	Calytriplex obovata Ruiz & Pav.
	Capraria monnieria Roxb
	Gratiola monnieri (L.) L.
	Gratiola portulacacea Weinm.
	Gratiola tetrandra Stokes
	Habershamia cuneifolia (Michx.) Raf.
	Herpestis cuneifolia Michx.
	Herpestis micromonnieria Griseb.
	Herpestis monnieri (L.) Rothm.
	Herpestis monnieri (L.) Kunth
	Herpestis procumbens Spreng.
	Limosella calycina Forssk.
	Lysimachia monnieri L.
	Monniera cuneifolia Michx
	Septas repens Lour
Common name (en)	Water hyssop
Common name (de)	Kleines Fettblatt
Common name (es)	Hisopo de agua
Common name (fr)	Hysope d’eau
Common name (hi)	ब्राह्मी
Common name (ja)	オトメアゼナ
Common name (ru)	Бакопа Монье
Common name (th)	พรมมิ
Common name (vi)	Rau đắng biển
Common name (zh)	假馬齒莧

Related herbal drug(s)

Centella asiatica (L.) Urb

Sample preparation	To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge
Mobile phase	Dichloromethane, methanol, water 14:6:1 (v/v/v)
Derivatization	Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min
System Suitability Test (SST)	Under development
Specific marker(s)	Under white light, after derivatization madecassoside: a greenish zone at R_F ~ 0.19 asiaticoside: a blue-violet zone at R_F ~ 0.28 bacopaside I: a red-violet zone at R_F ~ 0.31 bacopaside II: a red-violet zone at R_F ~ 0.44 bacoside A3: a violet zone at R_F ~ 0.44 bacopasaponin C: a purple-red zone at R_F ~ 0.47 bacopaside X: a red-violet zone at R_F ~ 0.48 madecassic acid: a blue zone at R_F ~ 0.70 asiatic acid: a blue zone at R_F ~ 0.77 bacosine: a pink zone at R_F ~ 0.87
HPTLC Chromatograms (different origin(s))
HPTLC Chromatograms (related drug(s))

Sample preparation	Mix 1.0 g of powdered sample with 10 mL of ethanol (96%) and sonicate for 10 min, then centrifuge the solution and use the supernatants as test solutions
Mobile phase	Dichloromethane, methanol, water 14:6:1 (v/v/v)
Derivatization	Derivatize with 10% sulfuric acid reagent, then heat the plate at 120°C for 3 min
HPTLC Chromatograms (evaluated method)

Sample preparation	Reflux 2 g of coarsely powdered substance under examination with 25 mL methanol for 15 minutes, cool and filter. Reflux the residue further with 2 x 25 mL of methanol, cool and filter. Combine all the filtrates and concentrate under vacuum to 25 mL
Mobile phase	Chloroform, methanol 7:3 (v/v)
Derivatization	Derivatize with 20% sulfuric acid reagent, then heat the plate at 100°C for 5-10 min
HPTLC Chromatograms (evaluated method)

Sample preparation	Add 10 mL of methanol to 0.5 g of the herbal drug. Sonicate for 10 min and centrifuge. Use the supernatant
Mobile phase	Dichloromethane, methanol, water 14:6:1 (v/v/v)
Derivatization	Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3-5 min
System Suitability Test (SST)	Under white light, after derivatization Bacopaside II: a purple-red zone at R_F ~ 0.44 bacopasaponin C: a purple-red zone at R_F ~ 0.47
Specific marker(s)	Under white light, after derivatization madecassoside: a greenish zone at R_F ~ 0.19 asiaticoside: a blue-violet zone at R_F ~ 0.28 bacopaside I: a red-violet zone at R_F ~ 0.31 bacopaside II: a red-violet zone at R_F ~ 0.44 bacoside A3: a violet zone at R_F ~ 0.44 bacopasaponin C: a purple-red zone at R_F ~ 0.47 bacopaside X: a red-violet zone at R_F ~ 0.48 madecassic acid: a blue zone at R_F ~ 0.70 asiatic acid: a blue zone at R_F ~ 0.77 bacosine: a pink zone at R_F ~ 0.87
HPTLC Chromatograms (evaluated method)

Sample preparation	Extract 1 g powdered drug with 100 mL of ethanol in a soxhlet apparatus consecutively three times. Filter and concentrate the combined extracts under vacuum and make up the volume to 10 mL with ethanol
Mobile phase	Butanol, acetic acid, water 4:1:5 (v/v/v)
Derivatization	Derivatize with vanillin sulfuric acid reagent, then heat the plate at 105°C
HPTLC Chromatograms (evaluated method)

Sample preparation	Methanolic extract
Mobile phase	Toluene, ethyl acetate, methanol, acetic acid 3:4:3:1 (v/v/v/v)
Derivatization	Derivatize with 20% vanillin sulfuric acid reagent, then heat the plate at 105°C for 5 min
HPTLC Chromatograms (evaluated method)

Sample preparation	Transfer about 2.5 g of powdered sample, accurately weighed, to a 100 mL round bottom flask fitted with a reflux condenser. Add 25 mL methanol on a water bath for 10 min, cool to room temperature, and decant the supernatant. Repeat until the last extract is colorless. Combine the extracts, filter, concentrate under vacuum, and adjust he volume to 100 mL using methanol. Before injection, pass through a membrane filter of 0.45 µm or finer pore size, discarding the first 5 mL of the filtrate
Mobile phase	Ethyl acetate, methanol, water 7:2:1 (v/v/v)
Derivatization	Derivatize with vanillin sulfuric acid reagent, then heat the plate at 70°C for 5-10 min
HPTLC Chromatograms (evaluated method)