Botanical Information Schisandra sphenanthera Rehder & E.H.Wilson (under development)
| Synonyms | Schisandra chinensis var. rubriflora Franch. |
| Schisandra flaccidiramosa C.R.Sun | |
| Schisandra grandiflora var. rubriflora (Franch.) C.K.Schneid. | |
| Common name (zh) | 南五味子 |
| Sample preparation | To 2.5 g of the powdered herbal drug add 10 mL of methanol. Extract at 25 °C in an ultrasonic bath for 5 min and centrifuge |
| Mobile phase | Acetic acid, ethyl acetate, toluene 2:22:46 (v/v/v) |
| Derivatization | Spray with a 100 g/L solution of sulfuric acid in methanol and heat in an oven at 120 °C for 7 min |
| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 50-mL conical flask, then add 10 mL of ethanol. Sonicate (560 W) the mixture for 1 h. Filter and transfer the filtrate to a 50-mL round bottomed flask. Evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 1 mL of ethanol |
| Mobile phase | Petroleum ether (60-80° C) , ethyl acetate, formic acid 15:5:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1 g of the powder, add 10 mL of cyclohexane, ultrasonicate for 30 min, filter, evaporate the filtrate to dryness. Dissolve the residue in 2 mL of methanol, centrifuge, evaporate the supernatant to dryness, and dissolve the residue in 1 mL of cyclohexane as the test solution |
| Mobile phase | Chloroform, acetone 60:1 (v/v) |
| Derivatization | Spray with phosphomolybdic acid reagent (dissolve 2 g of phosphomolybdic acid in 20 mL of water, add gently 30 mL of sulfuric acid, mix well), and heat at 105 °C until the spots appear |
| HPTLC Chromatograms |
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| Sample preparation | Add 1.0 g of powdered sample to 10 mL of methanol, sonicate for 30 min, filter and use the filtrate |
| Mobile phase | Ethyl acetate, hexane, acetic acid 10:10:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | 250 mg/mL of Schisandra chinensis fruit powder sonicated for 10 min and centrifuged or filtered |
| Mobile phase | Toluene, ethyl acetate, acetone, methanol 50:25:25:3 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol and heat at 100°C for 5 min |
| HPTLC Chromatograms |
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