Botanical Information Piper nigrum L.
| Synonyms | Muldera multinervis MiQ. |
| Muldera wightina MiQ. | |
| Piper aromaticum Lam. | |
| Piper trioicum Roxb. | |
| Common name (en) | Black pepper |
| Common name (de) | Pfeffer |
| Common name (fr) | Poivre noir |
| Common name (hi) | काली मिर्च |
| Common name (id) | Lada |
| Common name (it) | Pepe |
| Common name (ja) | コショウ |
| Common name (ko) | 후추 |
| Common name (nl) | Peper |
| Common name (pt) | Pimenta-preta |
| Common name (ru) | Перец чёрный |
| Common name (th) | พริกไทย |
| Common name (vi) | Hồ tiêu |
| Common name (zh) | 黑胡椒 |
| Sample preparation | |
| Mobile phase | |
| Derivatization |
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Cyclohexane, ethyl acetate 5:3 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
Image comparison of Piper nigrum Image comparison of Piper nigrum and related herbal drugs ATLAS Method document of Piper nigrum Selection of the ATLAS method of Piper nigrum visionCATS data (CAMAG) |
| Sample preparation | Alcoholic extract |
| Mobile phase | Toluene, ethyl acetate 7:1 (v/v) |
| Derivatization | Spray with dragendorff reagent followed by 5% methanolic sulfuric acid reagent |
| Sample preparation | Alcoholic extract |
| Mobile phase | Toluene, ethyl acetate 7:3 (v/v) |
| Derivatization | Spray with a solution of vanillin in sulfuric acid and heat at 110 °C for 10 min |
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Cyclohexane, ethyl acetate 5:3 (v/v) |
| Derivatization | Spray with anisaldehyde reagent, and heat the plate at 100°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | Weigh 1.0 g of the powdered sample and place it in a 50-mL conical flask wrapped in aluminium foil, then add 10 mL of ethanol. Sonicate (220 W) the mixture for 30 min. Filter the mixture. Freshly prepare the test solution |
| Mobile phase | n-hexane, ethyl acetate, acetone 8:4:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 2 g of powder, add 50 mL of methanol and reflux for 15 minutes, cool, filter. Reflux the residue further for two times with 75 mL methanol, cool and filter. Combine all the filtrates and concentrate under vacuum to 50 mL |
| Mobile phase | Benzene, ethyl acetate, diethyl ether 60:30:10 (v/v/v) |
| Derivatization | Spray the plate with vanillin sulfuric acid reagent. Heat the plate at 100°C for 5-10 minutes |
| Sample preparation | Weigh about 1 g of P. nigrum dried fruit powder in a 250 mL round bottom flask and add 20 mL of ethanol. Sonicate the mixture for 10 min at 60ºC. Filter the solution and evaporate to dryness on water bath. Reconstitute with 1 mL methanol and use as test solution |
| Mobile phase | Hexane, ethyl acetate, dichloromethane 5:3:2 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 0.5 g of the powder add 5 mL of dehydrated ethanol, ultrasonicate for 30 min and filter. Use the filtrate as test solution |
| Mobile phase | Benzene, ethyl acetate, acetone 7:2:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat under a current hot air to the spot distinct |
| HPTLC Chromatograms |
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| Sample preparation | Extract 1 g of powdered drug with 100 mL of ethanol in a soxhlet apparatus consecutively 3 times. Filter and concentrate the combined extract under vacuum and make up the volume to 10 mL with ethanol |
| Mobile phase | Toluene, ethyl acetate 7:3 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | Alcoholic extract |
| Mobile phase | Toluene, ethyl acetate 7:1 (v/v) |
| Derivatization | Spray with Dragendorff reagent followed by 5% methanolic sulfuric acid reagent |
| Sample preparation | Alcoholic extract |
| Mobile phase | Toluene, ethyl acetate 7:3 (v/v) |
| Derivatization | Spray with a solution of vanillin in sulfuric acid and heat at 105 °C for 5 min |
| Sample preparation | To 1.0 g of the powder add 10 mL of methanol, ultrasonicate for 30 min, cool, filter and use the filtrate as test solution |
| Mobile phase | Toluene, ethyl acetate, acetone 7:2:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat until the spots become visible. |
| Sample preparation | Macerate 500 mg of sample with 5 mL of chloroform for 10 minutes and shaking frequently. Filter and evaporate the filtrate to dryness. Dissolve the residue in 1 mL of chloroform |
| Mobile phase | n-Hexane, ethyl acetate 6:4 (v/v) |
| Derivatization | Spray the plate with acetic potassium iodobismuthate |
| HPTLC Chromatograms |
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| Sample preparation | Macerate 500 mg of sample with 5 mL of chloroform for 10 minutes and shaking frequently. Filter and evaporate the filtrate to dryness. Dissolve the residue in 1 mL of chloroform |
| Mobile phase | n-Hexane, ethyl acetate 6:4 (v/v) |
| Derivatization | Spray with 50% sulfuric acid reagent, dry in air for 15 minutes and heat the plate at 110°C until colors are fully developed |
| HPTLC Chromatograms |
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| Sample preparation | Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 min, then centrifuge the solution and use the supernatants as test solutions |
| Mobile phase | Hexane, ethyl acetate 5:3 (v/v) |
| Derivatization | Spray with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | To 0.5 g of the powder add 5 mL of dehydrated ethanol, ultrasonicate for 30 min and filter. Use the filtrate as test solution |
| Mobile phase | Benzene, ethyl acetate, acetone 7:2:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat under a current hot air to the spot distinct |
| HPTLC Chromatograms |
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