Botanical Information Piper longum L.
| Synonyms | Chavica longa H. Karst. |
| Chavica roxburghii Miq. | |
| Chavica sarmentosa Miq. | |
| Common name (en) | Long pepper |
| Common name (de) | Langer Pfeffer |
| Common name (es) | Pimienta larga |
| Common name (fr) | Poivrier long |
| Common name (hi) | पिप्पली |
| Common name (it) | Pepe lungo |
| Common name (ja) | ヒハツ |
| Common name (ko) | 필발 |
| Common name (pt) | Pimenta-longa |
| Common name (ru) | Перец длинный |
| Common name (th) | พริกไทยยาว |
| Common name (vi) | Tiêu lốt |
| Common name (zh) | 荜拔 |
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Cyclohexane, ethyl acetate 5:3 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
ATLAS Method document of Piper longum Image comparison of Piper longum Image comparison of Piper longum and related herbal drugs Selection of the ATLAS method of Piper longum visionCATS data |
| Sample preparation | To 0.5 g of the powder add 5 mL of methanol, sonicate for 10 min, centrifuge and use the supernatant |
| Mobile phase | Ethyl acetate, cyclohexane 30:50 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | Reflux 2 g of the coarsely powdered substance with 50-75 mL of methanol for 15 minutes, cool and filter. Wash the residue with 10 mL of methanol. Combine all the filtrates and concentrate to 10 mL |
| Mobile phase | Benzene, ethyl acetate, diethyl ether 60:30:10 (v/v/v) |
| Derivatization | Spray with vanillin-sulfuric acid reagent and heat the plate at 100°C for 5-10 minutes |
| HPTLC Chromatograms |
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| Sample preparation | To 0.8 g of the powder, add 5 mL of dehydrated ethanol, ultrasonicate for 30 min and filter. Use the filtrate as test solution |
| Mobile phase | Toluene, ethyl acetate, acetone 7:2:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat under a current hot air to the spot distinct |
| HPTLC Chromatograms |
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| Sample preparation | 20 g of powdered drug in 150 mL of hexane in a soxhlet apparatus to defeat the material. Further, extract the drug with methanol for 8 to 10 hours. Remove the solvent under reduced pressure. Dissolve 20 mg of methanolic extractive in 1 mL of methanol and use the solution |
| Mobile phase | Toluene, diethyl ether, dioxane 62.5:21.5:16 (v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | 1 g of powdered drug in 5 mL ethanol (96%), shake for 30 min, filter and use the filtrate as test solution |
| Mobile phase | Toluene, ethyl acetate, acetone 7:2:1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat the plate at 110°C until the colors appear |
| HPTLC Chromatograms |
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