Botanical Information Artemisia absinthium L. (procuring samples)

Synonyms Absinthium vulgare Lam.
Artemisia absinthia St.-Lag.
Artemisia absinthium var. insipida Stechm.
Artemisia arborescens f. rehan (Chiov.) Chiov.
Artemisia arborescens var. cupaniana Chiov.
Artemisia baldaccii Degen
Artemisia inodora Mill.
Artemisia kulbadica Boiss. & Buhse
Artemisia rehan Chiov.
Artemisia rhaetica Brügger
Common name (en) Wormwood
Common name (ar) شيح ابن سينا
Common name (de) Wermutkraut
Common name (es) Ajenjo
Common name (fr) Absinthe
Common name (ja) ニガヨモギ
Common name (ko) 향쑥
Common name (nl) Absintalsem
Common name (pt) Absinto
Common name (ru) Полынь горькая
Common name (zh) 中亚苦蒿

Sample preparation Place 2 g of the powdered herbal drug in 50 mL of boiling water and allow to stand for 5 min, shaking the flask several times. After cooling, add 5 mL of a 100 g/L solution of lead acetate. Mix and filter. Rinse the flask and the residue on the filter with 20 mL of water. Shake the filter with 50 mL of methylene chloride. Separate the organic layer, dry over anhydrous sodium sulfate, filter and evaporate the filtrate to dryness on a water-bath. Dissolve the residue in 0.5 mL of ethanol (96 %)
Mobile phase Acetone, glacial acetic acid, toluene, methylene chloride 10:10:30:50 (v/v/v/v)
Derivatization Spray with acetic anhydride - sulfuric acid solution R and heat at 100-105 °C for 5 min