1. High-Performance Thin-Layer Chromatography and Morpho-Anatomy of Monteverdia Ilicifolia “Espinheira-Santa” and its Adulterants, Wilmer Perera, CAMAG Scientific, Inc.
   
   
2. Hair, Hair Follicle, and Sebum Lipids Evaluation Using HPTLC, Ernesta Malinauskyte*, Katerin Mateo, TRI Princeton.
   
   
3. Psilocybe: Potency of Active Compounds, Psilocybin and Psilocin. A Single Lab Validation Using HPTLC, LC/MS/MS, Sidney Sudberg, Alkemist Labs.
   
   
4. HPTLC 4.0 - The Future of Planar Chromatography?, Eike Reich, HPTLC Association and delivery by Wilmer Perera, CAMAG Scientific. Inc.
   
   
5. HPTLC Separation of Novel Psychoactive Substances, Thomas Brettell, Cedar Crest College, Department of Chemical, Physical, & Forensic Sciences, 100 College Dr., Allentown, PA 18104, Marianne Staretz

1.    Abstract

Monteverdia ilicifolia (Mart. ex Reissek) Biral (syn. Maytenus ilicifolia), commonly known as “espinheira-santa”, is widely used in South American folk medicines to treat gastritis and ulcers. Several herbal products containing the leaves of M. ilicifolia are sold on the market. Many other species with similar leaf morphology are also called espinheira-santa and used for the same purpose. The most common adulterants that show morphological similarities to M. ilicifolia are Monteverdia aquifolia (Mart.) Biral [Celastraceae], Sorocea bonplandii (Baill.) W.C.Burger, Lanj. & Wess.Boer, [Moraceae], Zollernia ilicifolia Vogel [Fabaceae], Jodina rhombifolia (Hook & Arn.) Reissek (recognized as espinheira-de-três-pontas) [Santalaceae], and Citronella gongonha (Mart) R.A.Howard [Cardiopteridaceae]. This study aimed to differentiate M. ilicifolia from its adulterants by morphological, microscopic and HPTLC techniques. The morpho-anatomical studies of the leaves and stems of M. ilicifolia and its adulterant species have revealed noteworthy features that can help species identification. In addition, the comprehensive HPTLC analysis enables unambiguous identification of M. ilicifolia and quality control of commercial espinheira-santa.

2.    Abstract

Lipids are responsible for the maintenance of the structural integrity of skin and hair. We utilize silica gel high-performance thin-layer chromatography (HPTLC) to separate the lipids and densitometry to quantify the levels of free sterols, free fatty acids, ceramides, triacylglycerol, and squalene in hair, hair follicle, and sebum. The talk will reveal differences amongst them, challenges, and solutions for such analyses.

3.    Abstract

Psilocybe mushrooms have been in the news lately reflecting a growing interest from the psychology sector for its potential therapeutic value with Post Traumatic Growth (PTG) and other psychological issues. In this presentation a HPTLC phytochemical constituent profile will be demonstrated, as well as the quantitation and validation of the analysis of the major active components, psilocybin and psilocin. The results of HPTLC quantitation will be compared with HPLC & LC/MS/MS data

4.    Abstract

With the commercial introduction of plates coated with fine particles by MERCK in the late 1970s, the foundation for the evolution of HPTLC was laid. Over the last four decades, significant progress has been seen in the development of instruments. Most recently, even fully automatic systems became available. The available tools -instrument and software - have supported scientific research of all aspects of planar chromatography, stimulated curiosity, and expanded the range of what is possible. Yet, it seems that it was the discussion of a rigorous concept of standardization, its subsequent implementation into pharmacopoeias, and the resulting enforcement by authorities, which made HPTLC a distinct and accepted technique for routine analysis, particularly in quality control of herbal materials. What will be next? This presentation connects fundamental concepts, such as standardization and comprehensive HPTLC fingerprinting, with current ideas about the evaluation of system suitability, comparison of data across multiple plates, and the use of complementary developing solvents, leading to HPTLC 4.0. This vision may be developed into a global platform for collaboration of researchers and routine users of HPTLC. It has the potential of taking the inherent advantages of planar chromatography into a bright future.

5.    Abstract

In attempts to circumvent laws, clandestine laboratories modify the chemical structures of common drugs of abuse. This results in the synthesis of several different classes of novel psychoactive substances (NPSs) which include synthetic cannabinoids, cathinones, benzodiazepines, cathinones, phencyclidine analogues, tryptamines, and several others. Historically, traditional thin-layer chromatography (TLC) has been used for the analysis of seized drugs. Using traditional TLC does not always provide a satisfactory level of sensitivity, resolution, or documentation to analyze these new compounds many of which may be closely related structural isomers/ analogues. This presentation will discuss the use of high-performance thin-layer chromatography (HPTLC) for the analysis of several classes of NPSs. A CAMAG automatic TLC system, HPTLC Silica gel 60 F254 20 x 10-cm plates and HPTLC-Platten 10 x 10-cm RP-18 WF254s plates were used in all analyses. A CAMAG TLC visualizer was used to visualize developed plates and take pictures with white light, 254 nm and 366 nm wavelengths. RF values demonstrated good intra- and inter-day reproducible CV%

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