Botanical Information Berberis aristata DC. (under development)
| Synonyms | Berberis bussmul K.Koch ex Miq. |
| Berberis ceratophylla G.Don | |
| Berberis chitria D.Don | |
| Berberis chitria var. sikkimensis C.K.Schneid. | |
| Berberis coccinea K.Koch | |
| Berberis coerulescens G.Nicholson | |
| Berberis elegans K.Koch | |
| Berberis gracilis Lindl. | |
| Berberis gracillima K.Koch | |
| Berberis macrophylla K.Koch | |
| Berberis serratifolia K.Koch | |
| Berberis sikkimensis (C.K.Schneid.) Ahrendt | |
| Berberis sikkimensis var. baileyi Ahrendt J. Linn. | |
| Berberis sikkimensis var. glabramea Ahrendt | |
| Berberis umbellata Lindl. | |
| Berberis undulata K.Koch | |
| Common name (en) | Indian barberry |
| Common name (de) | Begrannte Berberitze |
| Common name (hi) | दारुहरिद्रा |
| Sample preparation | Extract about 1 g, accurately weighed, powdered drug with 10 mL of methanol by heating on a water bath for 10 min with intermittent shaking. Filter and use the filtrate |
| Mobile phase | n-propanol, formic acid (85%), water 9:01:0.9 (v/v/v) |
| Derivatization | Spray with Dragendorff reagent |
Related herbal drug(s)
Berberis aristata DC. (under development)
Berberis asiatica Roxb. ex DC. (procuring samples)
Berberis vulgaris L. (under development)
Coptis chinensis Franch. (under development)
Coptis japonica (Thunb.) Makino (under development)
Coscinium fenestratum (Gaertn.) Colebr. (procuring samples)
Hydrastis canadensis L. (under development)
| Sample preparation | To 250 mg of the powdered herbal drug add 4 mL of a mixture of 20 volumes of water and 80 volumes of methanol. Sonicate for 10 min and filter. Wash the residue with 2 quantities, each of 2 mL, of methanol. Combine the solutions and dilute to 20 mL with methanol |
| Mobile phase | Formic acid, water, ethyl acetate 10:10:80 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1 g of the coarsely powdered substance, add 40 mL of methanol, reflux for 15 minutes, cool and filter. Reflux the residue further for two times with 25 mL of methanol, cool and filter. Combine all the filtrates and concentrate under vacuum to 25 mL |
| Mobile phase | Ethyl acetate, formic acid, acetic acid, water 80:10:10:20 (v/v/v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1 g of the powder add 20 mL of methanol, ultrasonicate for 20 minutes, filter and use the filtrate as test solution |
| Mobile phase | Upper layer of a mixture of n-butanol, acetic acid, water 2:0.5:1 (v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
|
Related herbal drug(s)
Berberis aristata DC. (under development)
Berberis asiatica Roxb. ex DC. (procuring samples)
Berberis vulgaris L. (under development)
Coptis chinensis Franch. (under development)
Coptis japonica (Thunb.) Makino (under development)
Coscinium fenestratum (Gaertn.) Colebr. (procuring samples)
Hydrastis canadensis L. (under development)
| Sample preparation | To 250 mg of the powdered herbal drug add 4 mL of a mixture of 20 volumes of water and 80 volumes of methanol. Sonicate for 10 min and filter. Wash the residue with 2 quantities, each of 2 mL, of methanol. Combine the solutions and dilute to 20 mL with methanol |
| Mobile phase | Formic acid, water, ethyl acetate 10:10:80 (v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
|
| Sample preparation | Shake 1 g of powdered substance with 10 mL of methanol for 10 to 15 min and filter. Wash the residue with 5 mL of methanol, add washing to the filtrate |
| Mobile phase | n-butanol, acetic acid, water 70:10:20 (v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
|
| Sample preparation | Extract 20 g of powdered drug in Soxhlet apparatus with n-hexane (150 mL) to defat the drug material (5 to 7h) and further extract with 150 mL of methanol (8 to 9h). Filter and concentrate the methanolic extract in vacuum. Take 2 mg of residue and dissolve in 1 mL of methanol |
| Mobile phase | Butanol, ethyl acetate, acetic acid, water 3:5:1:1 (v/v/v/v) |
| Derivatization | Spray with Dragendorff reagent |