Botanical Information Chamaemelum nobile (L.) All. (under development)
| Synonyms | Anacyclus aureus L. |
| Anthemis nobilis L. | |
| Anthemis nobilis var. aurea (L.) | |
| Anthemis nobilis var. discoidea (Boiss. ex Willk.) | |
| Anthemis santolinoides Munby | |
| Chamaemelum nobile f. discoideum (Boiss. ex Willk.) | |
| Chamaemelum nobile var. discoideum (Boiss. ex Willk.) | |
| Common name (en) | Roman chamomile |
| Common name (de) | Römische Kamille |
| Common name (fr) | Camomille romaine |
| Common name (ja) | ローマンカモミール |
| Common name (ru) | Ромашка римская |
| Common name (zh) | 果香菊 |
| Sample preparation | 1.0 g of powdered herbal drug in 10 mL of methanol, sonicate for 10 min and centrifuge. The supernatant is used as test solution |
| Mobile phase | Ethyl acetate, formic acid, water 80:10:10 (v/v/v) |
| Derivatization | Heat at 100°C for 3 min, let the plate cool down, derivatize with the mixture (NP/PEG reagents), and dry the plate for 2 min in a cold air flow |
| System Suitability Test (SST) | Under development |
| Sample preparation | 1.0 g of powdered herbal drug in 10 mL of methanol, sonicate for 10 min and centrifuge. The supernatant is used as test solution |
| Mobile phase | Cyclohexane, ethyl acetate 1:1 (v/v) |
| Derivatization | Derivatize with anisaldehyde sulfuric acid reagent, then heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Sample preparation | To 0.5 g of the powdered herbal drug add 5.0 mL of methanol. Sonicate for 15 min, then filter or centrifuge the solution and use the filtrate or supernatant |
| Mobile phase | Ethyl acetate, formic acid, water 8:1:1 (v/v/v) |
| Derivatization | Heat at 100-105 °C for 5 min. Spray the warm plate with a 10 g/L solution of diphenylboric acid aminoethyl ester in methanol, then with a 50 g/L solution of macrogol 400 in methanol or, alternatively, dip the warm plate in a 5 g/L solution of diphenylboric acid aminoethyl ester in ethyl acetate, then in a 50 g/L solution of macrogol 400 in methylene chloride. Allow the plate to dry in air for about 1 min and examine in ultraviolet light at 366 nm |
| HPTLC Chromatograms |
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| Sample preparation | 100 mg/mL of powdered drug in methanol, sonicated for 10 min, and centrifuged. Use the clear supernatant |
| Mobile phase | Cyclohexane, ethyl acetate 1:1 (v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 100°C for 3 min |
| HPTLC Chromatograms |
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