Sample preparation |
Use 30 g of whole herbal drug, a 1000 mL flask, 500 mL of water as distillation liquid and 0.50 mL of 1,2,4-trimethylbenzene in the graduated tube. Distil at a rate of 3-4 mL/min for 4 h. Towards the end of this period, stop the flow of water to the condenser assembly but continue distilling until the blue, steam-volatile components have reached the lower end of the condenser. Immediately re-start the flow of water to the condenser assembly to avoid warming the separation space. Stop the distillation after a further 10 min. Dilute 50 µL of essential oil in 1 mL of xylene |
Mobile phase |
Toluene, ethyl acetate 95:5 (v/v) |
Derivatization |
Spray with anisaldehyde solution and heat at 100-105°C for 5-10 min |
System Suitability Test (SST) |
According to the monograph |
HPTLC Chromatograms (evaluated method) |
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