Botanical Information Angelica sinensis (Oliv.) Diels
| Synonyms | Angelica polymorpha var. sinensis Oliv. Hooker's |
| Common name (en) | Dong quai |
| Common name (ar) | حشيشة الملاك الصينية |
| Common name (de) | Chinesische Engelwurz |
| Common name (fr) | Angélique de Chine |
| Common name (th) | ตังกุย |
| Common name (vi) | Đương quy |
| Common name (zh) | 当归 |
| Sample preparation | Mix 1.0 g of powdered sample with 5.0 mL of methanol and shake for 10 min, then centrifuge the solution and use the supernatant as test solution |
| Mobile phase | Toluene, ethyl acetate, acetic acid 90:10: 1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol, then heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under UV 366 nm prior to derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
ATLAS Method document Image comparison of Angelica sinensis Image comparison of Angelica sinensis and related herbal drugs visionCATS data (CAMAG software) |
| Sample preparation | To 1 g of the freshly powdered herbal drug add 4 mL of heptane, close and sonicate for 5 min. Centrifuge the mixture and use the supernatant |
| Mobile phase | Acetic acid, ethyl acetate, toluene 1:10:90 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Weigh 1.0 g of the powdered sample and put into a 50 mL centrifugal tube, then add 10 mL of diethyl ether. Close the cap of the tube, shake occasionally for 2 h and centrifuge at about 1200 x g for 10 min. Transfer the supernatant to a new tube and evaporate to dryness on a warm water bath maintained at a temperature below 50°C. Dissolve the residue in 1 mL of ethyl acetate |
| Mobile phase | hexane, ethyl acetate 5:1 (v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol anf heat at 110°C for 10 min |
| Sample preparation | To 0.5 g of the powder add 20 mL of ether, ultrasoniate for 10 min and filter. Evaporate the filtrate to dryness and dissolve the residue in 1 mL of ethanol as the test solution |
| Mobile phase | Hexane, ethyl acetate 4:1 (v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | To 3 g of the powder add 50 mL of 1% solution of sodium bicarbonate, ultrasonicate for 10 minutes, and centrifuge. Adjust the supernatant to pH 2 ~ 3 with dilute hydrochloric acid, extract by shaking with two 20 mL quantities of ether, combine the ether extracts and evaporate to dryness. Dissolve the residue in 1 mL of methanol as the test solution |
| Mobile phase | Cyclohexane, dichloromethane, ethyl acetate, formic acid 4:1:1:0.1 (v/v/v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | Add 1.0 g of powdered sample to 10 mL of methanol, ultrasonicate for 30 min filter and use the filtrate |
| Mobile phase | Ethyl acetate, petroleum ether (30-60°C) 15:85 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | Reflux 1 g of the powdered sample with 10 mL of dichloromethane for 30 minutes and filtering. Evaporate the filtrate to dryness. Dissolve the residue in 1 mL of toluene |
| Mobile phase | Toluene, ethyl acetate, formic acid 86:14:5 (v/v/v) |
| Derivatization | Spray with anisaldehyde reagent and heat at 105°C for 10 min |
| Sample preparation | 5 g coarsely ground drug are soxhlet-extracted with 50 mL n-hexane for one hour. The extract is evaporated to dryness and redissolved in 2.5 mL ethanol and filtered |
| Mobile phase | Toluene, ethyl acetate, acetic acid 90:10:1 (v/v/v) |
| Derivatization | Spray with anisaldehyde reagent, and heat the plate at 100°C for 5-10 min |