Botanical Information Angelica pubescens Maxim.
| Synonyms | Angelica myriostachys Koidz. |
| Angelica polyclada Franch. | |
| Angelica schishiudo Koidz. | |
| Common name (en) | Du huo |
| Common name (es) | Shishiudo |
| Common name (vi) | Độc hoạt |
| Common name (zh) | 猪独活 |
| Sample preparation | Mix 1.0 g of powdered sample with 5.0 mL of methanol and shake for 10 min, then centrifuge the solution and use the supernatant as test solution |
| Mobile phase | Toluene, ethyl acetate, acetic acid 90:10: 1 (v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in methanol, then heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under UV 366 nm prior to derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
ATLAS Method document Image comparison of Angelica pubescens Image comparison of Angelica pubescens and related herbal drugs visionCATS data (CAMAG software) |
| Sample preparation | To 1 g of the freshly powdered herbal drug add 4 mL of heptane, close and sonicate for 5 min. Centrifuge the mixture and use the supernatant |
| Mobile phase | Acetic acid, ethyl acetate, toluene 1:10:90(v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Weigh 2.0 g of the powdered sample and put into a 50-mL conical flask, then add 10 mL of diethyl ether. Allow to stand overnight. Filter and transfer the filtrate to another conical flask. Evaporate to dryness on a water bath at 35?C. Dissolve the residue in 2 mL of dichloromethane |
| Mobile phase | n-hexane, toluene, ethyl acetate 2:1:1 (v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of ethanol. Ultrasonicate for 15 min, filter and use the filtrate as test solution |
| Mobile phase | Petroleum ether (60-90°C), ethyl acetate 7:3 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | Add 1.0 g of the powdered sample to 10 mL of methanol, ultrasonicate for 15 minutes, filter and use the filtrate |
| Mobile phase | n-Hexane, ethyl acetate 2:1 (v/v) |
| Derivatization | No derivatization |
| Sample preparation | 5 g of powdered drug are soxhlet-extracted with 50 mL dichloromethane for 3h. The extract is evaporated to dryness. 100 mg of the residue are dissolved in 10.0 ethanol absolute |
| Mobile phase | Toluene, ethyl acetate 80:20 (v/v) |
| Derivatization | Spray with anisaldehyde-sulfuric acid reagent and heat at 10°C for 5-10 min |
| Sample preparation | 5 g of powdered drug are soxhlet-extracted with 50 mL hexane for 3h. The extract is evaporated to dryness. 100 mg of the residue are dissolved in 10.0 ethanol absolute |
| Mobile phase | Toluene, ethyl acetate 80:20 (v/v) |
| Derivatization | Spray with anisaldehyde-sulfuric acid reagent and heat at 10°C for 5-10 min |