HPTLC ATLAS

 

Botanical Information Angelica pubescens Maxim.

Synonyms Angelica myriostachys Koidz.
Angelica polyclada Franch.
Angelica schishiudo Koidz.
Common name (en) Du huo
Common name (es) Shishiudo
Common name (vi) Độc hoạt
Common name (zh) 猪独活

Sample preparation Mix 1.0 g of powdered sample with 5.0 mL of methanol and shake for 10 min, then centrifuge the solution and use the supernatant as test solution
Mobile phase Toluene, ethyl acetate, acetic acid 90:10: 1 (v/v/v)
Derivatization Spray with 10% sulfuric acid in methanol, then heat the plate at 100°C for 3 min
System Suitability Test (SST) Under UV 366 nm prior to derivatization
  • Imperatorin: green fluorescent zone RF ~ 0.33
  • Osthole: blue fluorescent zone RF ~ 0.38
  • Isoimperatorin: green fluorescent zone RF ~ 0.44
HPTLC Chromatograms (different origin(s))
HPTLC Chromatograms (related drug(s))
ATLAS documents ATLAS Method document
Image comparison of Angelica pubescens
Image comparison of Angelica pubescens and related herbal drugs
visionCATS data (CAMAG software)

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