HPTLC Association | HPTLC ATLAS

Amomum aromaticum Roxb.

HPTLC ATLAS

Botanical Information Amomum aromaticum Roxb.

Synonyms	Alpinia fasciculate (Roscoe) Steud.
	Amomum fasciculatum (Roscoe) Benth. & Hook. F. ex B.D.Jacks.
	Cardamomum aromaticum (Roxb.) Kuntze
	Geocallis fasciculate (Roscoe) Horan.
	Renealmia fasciculate Roscoe

Seed

Related herbal drug(s)

Alpinia hainanensis K. Schum.
Alpinia japonica (thumb.) Miq.
Alpinia oxyphylla Miq.
Amomum aculeatum Roxb.
Amomum compactum Sol. ex Maton
Amomum longiligulare T.L.Wu
Amomum tsao-ko Crevost & Lemarié
Amomum verum Blackw.
Amomum villosum Lour.
Amomum dealbatum Roxb. (procuring samples)
Amomum subulatum Roxb. (procuring samples)

ATLAS method (ATLAS Method)

Sample preparation	To 1 g of the powdered herbal drug add 5 mL of methylene chloride. Sonicate for 10 min. Centrifuge and use the supernatant
Mobile phase	Ethyl acetate, toluene 7:93 (v/v)
Derivatization	Spray with anisaldehyde solution and heat at 100°C for 3 min
System Suitability Test (SST)	Under development
Specific marker(s)	Under white light, after derivatization alpinetin: yellow zone R_F ~ 0.01 cardamomin: yellow zone R_F ~ 0.13 borneol: greyish-brown zone R_F ~ 0.19 1,8-cineol: bluish-violet zone R_F ~ 0.37 bornyl acetate: greyish-brown R_F ~ 0.52
HPTLC Chromatograms (different origin(s))
HPTLC Chromatograms (related drug(s))
ATLAS documents	ATLAS Method document of Amomum aromaticum Image comparison of Amomum aromaticum Image comparison of Amomum aromaticum and related herbal drugs visionCATS data (CAMAG software)

European Pharmacopoeia 9.8 for Amomum compactum

Sample preparation	To 1 g of the powdered herbal drug add 5 mL of methylene chloride. Sonicate for 10 min. Centrifuge and use the supernatant
Mobile phase	Ethyl acetate, toluene 7:93 (v/v)
Derivatization	Spray with anisaldehyde solution and heat at 100-105°C for 3 min
System Suitability Test (SST)	Under white light, after derivatization borneol: greyish-brown zone R_F ~ 0.19 1,8-cineol: bluish-violet zone R_F ~ 0.37 bornyl acetate: greyish-brown R_F ~ 0.52
HPTLC Chromatograms (evaluated method)

Quality Standards of Indian Medicinal Plants Vol. 13

Sample preparation	Macerate 20 g of the powdered drug with n-hexane (100 mL x 3) overnight each time . Filter and remove the solvent from the combined extract under reduced pressure to get the residue (0.783 g). Dissolve 20 mg of the residue in 10 ml of n-hexane
Mobile phase	Toluene, ethyl acetate 93:7 (v/v)
Derivatization	Spray with anisaldehyde reagent, and heat the plate at 105°C for 5 to 10 min
HPTLC Chromatograms (evaluated method)