HPTLC ATLAS

 

Botanical Information Amomum subulatum Roxb. (procuring samples)

Synonyms Cardamomum subulatum (Roxb.) Kuntze
Common name (en) Black cardamom
Common name (de) Schwarze Kardamom
Common name (fr) Grande cardamome
Common name (hi) बड़ी इलायची
Common name (ko) 향두구
Common name (ru) Чёрный кардамон
Common name (vi) Đậu khấu thơm
Common name (zh) 香豆蔻

Sample preparation Extract 5 g of the powdered drug with 30 mL of n-hexane in a soxhlet apparatus for 3 h. Evaporate the solvent under reduce pressure to get the residue (0.12 g). Dissolve 1 mg of the residue in 1 mL of n-hexane
Mobile phase Toluene, ethyl acetate, methanol 93:7:0.2 (v/v/v)
Derivatization Spray with anisaldehyde reagent, and heat the plate at 105°C for 5 to 10 min
Sample preparation Extract 2 g of the powdered drug with 15 mL of ethanol in a soxhlet apparatus for 18 h. Remove the solvent under reduce pressure. Dissolve the residue in 1 mL of ethanol
Mobile phase Toluene, ethyl acetate, formic acid 7:3:1 (v/v/v)
Derivatization Spray with vanillin-sulfuric acid reagent and heat the plate at 105°C for 5 min

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