Botanical Information Amomum tsao-ko Crevost & Lemarié
| Synonyms | Amomum hongtsaoko C.F.Liang & D.Fang |
| Common name (en) | Amomum tsao-ko |
| Common name (vi) | Thảo quả |
Related herbal drug(s)
Alpinia galanga (L.) Willd.
Alpinia hainanensis K. Schum.
Alpinia japonica (thumb.) Miq.
Alpinia oxyphylla Miq.
Amomum aculeatum Roxb.
Amomum aromaticum Roxb.
Amomum compactum Sol. ex Maton
Amomum longiligulare T.L.Wu
Amomum verum Blackw.
Amomum villosum Lour.
Amomum dealbatum Roxb. (procuring samples)
Amomum subulatum Roxb. (procuring samples)
| Sample preparation | To 1 g of the powdered herbal drug add 5 mL of methylene chloride. Sonicate for 10 min. Centrifuge and use the supernatant |
| Mobile phase | Ethyl acetate, toluene 7:93 (v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
ATLAS Method document of Amomum tsao-ko Image comparison of Amomum tsao-ko Image comparison of Amomum tsao-ko and related herbal drugs visionCATS data (CAMAG software) |
| Sample preparation | To 1 g of the powdered herbal drug add 5 mL of methylene chloride. Sonicate for 10 min. Centrifuge and use the supernatant |
| Mobile phase | Ethyl acetate, toluene 7:93 (v/v) |
| Derivatization | Spray with anisaldehyde solution and heat at 100-105°C for 3 min |
| System Suitability Test (SST) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| Sample preparation | Weigh 2.0 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 20 mL of methanol. Sonicate (140 W) the mixture for 30 min. Centrifuge at about 2800 × g for 10 min. Filter the supernatant |
| Mobile phase | n-hexane, acetone, ethyl acetate 30:1:0.5 (v/v/v) |
| Derivatization | Spray with anisaldehyde reagent (1 mL of sulfuric acid to 50 mL of glacial acetic acid and then add 0.5 mL of 4-methoxybenzaldehyde) and heat the plate at 105°C until the spot appear |
| HPTLC Chromatograms |
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| Sample preparation | Dissolve a quantity of volatile oil to produce a solution containing 50 µL/mL as the test solution |
| Mobile phase | n-hexane, ethyl acetate 17:3 (v/v) |
| Derivatization | Spray with 5% vanillin in sulfuric acid, heat at 105°C until the spots appear |
| Sample preparation | Distill a quantity of powdered sample with water, the volatile oil is produced. Take 0.5 mL of volatile oil and add ethanol to 10 mL |
| Mobile phase | n-Hexane, ethyl acetate 17:3 (v/v) |
| Derivatization | Spray with a 5% solution of vanillin in sulfuric acid and heat the plate at 105°C for 2 min |